PMID- 15196596
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1689
IP  - 2
DP  - 2004 Jun 28
TI  - Matrix proteoglycans are markedly affected in advanced laryngeal squamous cell 
      carcinoma.
PG  - 152-61
AB  - Proteoglycans (PGs) are implicated in the growth and progression of malignant 
      tumors. In this study, we examined the concentration and localization of PGs in 
      advanced (stage IV) laryngeal squamous cell carcinoma (LSCC) and compared with 
      human normal larynx (HNL). LSCC and HNL sections were examined 
      immunohistochemically with a panel of antibodies, and tissues extracts were 
      analyzed by biochemical methods including immunoblotting and high performance 
      liquid chromatography (HPLC). The results demonstrated significant destruction of 
      cartilage in LSCC, which was followed by marked decrease of aggrecan and link 
      protein. In contrast to the loss of aggrecan in LSCC, accumulation of versican 
      and decorin was observed in the tumor-associated stroma. Biochemical analyses 
      indicated that aggrecan, versican, decorin and biglycan comprise the vast 
      majority of total PGs in both healthy and cancerous tissue. In LSCC the absolute 
      amounts of KS/CS/DS-containing PGs were dramatically decreased about 18-fold in 
      comparison to HNL. This decrease is due to the loss of aggrecan. Disaccharide 
      analysis of CS/DSPGs from LSCC showed a significant reduction of 6-sulfated 
      Delta-disaccharides (Deltadi-6S) with a parallel increase of 4-sulfated 
      Delta-disaccharides (Deltadi-4S) as compared to HNL. The obtained data clearly 
      demonstrate that tumor progression is closely related to specific alteration of 
      matrix PGs in LSCC. The altered composition of PGs in cartilage, as well as in 
      tumor-associated stroma, is crucial for the biological behaviour of cancer cells 
      in the diseased tissue.
FAU - Skandalis, Spyros S
AU  - Skandalis SS
AD  - Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and 
      Natural Products, Department of Chemistry, University of Patras, 26500 Patras, 
      Greece.
FAU - Theocharis, Achilleas D
AU  - Theocharis AD
FAU - Theocharis, Dimitrios A
AU  - Theocharis DA
FAU - Papadas, Theodoros
AU  - Papadas T
FAU - Vynios, Demitrios H
AU  - Vynios DH
FAU - Papageorgakopoulou, Nickoletta
AU  - Papageorgakopoulou N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proteoglycans)
SB  - IM
MH  - Biomarkers, Tumor/*metabolism
MH  - Cells, Cultured
MH  - Extracellular Matrix/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Larynx/*metabolism/pathology
MH  - Neoplasm Staging
MH  - Neoplasms, Squamous Cell/*metabolism/pathology
MH  - Proteoglycans/*metabolism
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 2004/06/16 05:00
MHDA- 2004/08/11 05:00
CRDT- 2004/06/16 05:00
PHST- 2003/11/14 00:00 [received]
PHST- 2004/02/16 00:00 [revised]
PHST- 2004/03/12 00:00 [accepted]
PHST- 2004/06/16 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2004/06/16 05:00 [entrez]
AID - S0925443904000328 [pii]
AID - 10.1016/j.bbadis.2004.03.006 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2004 Jun 28;1689(2):152-61. doi: 
      10.1016/j.bbadis.2004.03.006.