PMID- 15196631
OWN - NLM
STAT- MEDLINE
DCOM- 20041110
LR  - 20151119
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 278
IP  - 2
DP  - 2004 Jul 8
TI  - Incorporation of ovalbumin into ISCOMs and related colloidal particles prepared 
      by the lipid film hydration method.
PG  - 263-74
AB  - The aim of this study was to investigate the incorporation of a model antigen, 
      fluorescently labelled ovalbumin (FITC-OVA), into various colloidal particles 
      including immune stimulating complexes (ISCOMs), liposomes, ring and worm-like 
      micelles, lamellae and lipidic/layered structures that are formed from various 
      combinations of the triterpene saponin Quil A, cholesterol and 
      phosphatidylethanolamine (PE) following hydration of PE/cholesterol lipid films 
      with aqueous solutions of Quil A. Colloidal dispersions of these three components 
      were also prepared by the dialysis method for comparison. FITC-OVA was conjugated 
      with palmitic acid (P) and PE to produce P-FITC-OVA and PE-FITC-OVA, 
      respectively. Both P-FITC-OVA and PE-FITC-OVA could be incorporated in all 
      colloidal structures whereas FITC-OVA was incorporated only into liposomes. The 
      incorporation of PE-FITC-OVA into all colloidal structures was significantly 
      higher than P-FITC-OVA (P < 0.05). The degree of incorporation of protein was in 
      the order: ring and worm-like micelles < liposomes and lipidic/layered structures 
      < ISCOMs and lamellae. The incorporation of protein into the various particles 
      prepared by the lipid film hydration method was similar to those for colloidal 
      particles prepared by the dialysis method (provided both methods lead to the 
      formation of the same colloidal structures). In the case of different colloidal 
      structures arising due to the preparation method, differences in encapsulation 
      efficiency were found (P < 0.05) for formulations with the same polar lipid 
      composition. This study demonstrates that the various colloidal particles formed 
      as a result of hydrating PE/cholesterol lipid films with different amounts of 
      Quil A are capable of incorporating antigen, provided it is amphipathic. Some of 
      these colloidal particles may be used as effective vaccine delivery systems.
FAU - Demana, Patrick H
AU  - Demana PH
AD  - Drug Delivery Solutions, New Zealand National School of Pharmacy, University of 
      Otago, Dunedin.
FAU - Davies, Nigel M
AU  - Davies NM
FAU - Berger, Bianca
AU  - Berger B
FAU - Rades, Thomas
AU  - Rades T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Colloids)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (ISCOMs)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Quillaja Saponins)
RN  - 0 (Saponins)
RN  - 39382-08-6 (phosphatidylethanolamine)
RN  - 66594-14-7 (Quil A)
RN  - 9006-59-1 (Ovalbumin)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Cholesterol/chemistry
MH  - Colloids
MH  - Drug Delivery Systems
MH  - Fluorescein-5-isothiocyanate/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - ISCOMs/*chemistry
MH  - Ovalbumin/administration & dosage/*chemistry
MH  - Phosphatidylethanolamines/chemistry
MH  - Quillaja Saponins
MH  - Saponins/chemistry
MH  - Technology, Pharmaceutical
EDAT- 2004/06/16 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/06/16 05:00
PHST- 2003/12/02 00:00 [received]
PHST- 2004/02/26 00:00 [revised]
PHST- 2004/03/11 00:00 [accepted]
PHST- 2004/06/16 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/06/16 05:00 [entrez]
AID - S037851730400198X [pii]
AID - 10.1016/j.ijpharm.2004.03.021 [doi]
PST - ppublish
SO  - Int J Pharm. 2004 Jul 8;278(2):263-74. doi: 10.1016/j.ijpharm.2004.03.021.