PMID- 15196634
OWN - NLM
STAT- MEDLINE
DCOM- 20041110
LR  - 20181130
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 278
IP  - 2
DP  - 2004 Jul 8
TI  - Indocyanine green-loaded biodegradable nanoparticles: preparation, 
      physicochemical characterization and in vitro release.
PG  - 293-301
AB  - PURPOSE: The objective of this study is to develop indocyanine green (ICG)-loaded 
      biodegradable nanoparticles by using biodegradable polymer, 
      poly(DL-lactic-co-glycolic acid) (PLGA). METHOD: PLGA nanoparticles entrapping 
      ICG were prepared by a modified spontaneous emulsification solvent diffusion 
      method. To optimize the nanoparticle formulation, the influence of formulation 
      parameters such as types of ICG, amount of ICG and the polymer were investigated. 
      The ICG entrapment in nanoparticles, nanoparticle size and zeta potential were 
      determined. The surface characterization was performed by atomic force microscopy 
      (AFM) and the release of ICG from nanoparticles was determined. RESULTS: All PLGA 
      nanoparticle formulations were found to have the mean diameter within the range 
      of 300-410 nm with polydispersity index (PI) within the range of 0.01-0.06. 
      Indocyanine green showed more efficient entrapment as compared to indocyanine 
      green sodium iodide salt. All indocyanine green-loaded nanoparticle formulations 
      were found to have almost similar ICG content of nanoparticles and showed 
      increase in ICG entrapment with increase in the amount of polymer. The ICG 
      entrapment reached 74% when ICG: PLGA weight ratio in the formulation reached 
      1:800. AFM images indicated that the nanoparticles were almost spherical in shape 
      and had numerous pores on their surfaces. The release pattern consisted of two 
      phases, with initial exponential phase releasing about 78% of ICG (within 8 h) 
      followed by a slow phase releasing about 2% of ICG (within next 16 h). 
      CONCLUSIONS: ICG-loaded PLGA nanoparticles were prepared and the formulation was 
      optimized. The increase in amount of polymer in formulation leads to higher ICG 
      entrapment. Nanoparticles formed were spherical and had porous surfaces and 
      exhibited the characteristic release pattern of a monolithic matrix based system.
FAU - Saxena, Vishal
AU  - Saxena V
AD  - Department of Pharmacy and Administrative Sciences, College of Pharmacy and 
      Allied Health Professions, St. John's University, 8000 Utopia Parkway, Jamaica, 
      NY 11439, USA.
FAU - Sadoqi, Mostafa
AU  - Sadoqi M
FAU - Shao, Jun
AU  - Shao J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Coloring Agents)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - IX6J1063HV (Indocyanine Green)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Chemistry, Pharmaceutical
MH  - Coloring Agents/administration & dosage/*chemistry
MH  - Drug Delivery Systems
MH  - Indocyanine Green/administration & dosage/*chemistry
MH  - Lactic Acid/*chemistry
MH  - Microspheres
MH  - Nanotechnology
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Solubility
MH  - Surface Properties
EDAT- 2004/06/16 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/06/16 05:00
PHST- 2003/07/17 00:00 [received]
PHST- 2004/01/06 00:00 [revised]
PHST- 2004/03/15 00:00 [accepted]
PHST- 2004/06/16 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/06/16 05:00 [entrez]
AID - S0378517304002261 [pii]
AID - 10.1016/j.ijpharm.2004.03.032 [doi]
PST - ppublish
SO  - Int J Pharm. 2004 Jul 8;278(2):293-301. doi: 10.1016/j.ijpharm.2004.03.032.