PMID- 15201029
OWN - NLM
STAT- MEDLINE
DCOM- 20040907
LR  - 20081121
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 101
IP  - 1
DP  - 2004 Jun 10
TI  - Genetics and geographical variation of porcine reproductive and respiratory 
      syndrome virus (PRRSV) in Thailand.
PG  - 9-21
AB  - The Thai isolates of porcine reproductive and respiratory syndrome virus (PRRSV) 
      were obtained from the Chulalongkorn University-Veterinary Diagnostic Laboratory 
      (CU-VDL). Virus isolation was confirmed by immunoperoxidase monolayer assay 
      (IPMA) using SDOW-17. The virus genotype was determined using nested multiplex 
      RT-PCR (nm RT-PCR) of ORF 1b. The nm RT-PCR was able to detect at least 
      10TCID50/ml of PRRSV. Of 137 Thai isolates, 66.42% belonged to the European (EU) 
      genotype and 33.58% to the North American (US) genotype. ORF5 products of the 
      eight US strains (00CS1, 01NP1, 01UD6, 02CB13, 02KK1, 02PB1, 02SP2 and 02SP3) and 
      the six EU strains (01CB1, 01RB1, 02BR1, 02CB12, 02SB2 and 03RB1) were sequenced 
      for genetic variation analysis. The US strains of the Thai isolates are clustered 
      within the same group and are more closely related to the IAF-EXP91 from Canada 
      (89-90% nucleotide identity), whereas the EU strains were very similar to the EU 
      prototype, Lelystad virus (87-97.5% nucleotide identity). The ORF5 nucleotide 
      identities within the US genotype tested in this study compared to the US 
      prototype, VR-2332 varied from 83.7 to 85.2%, whereas 83.5-85.5% amino acid 
      identities were found. Based on the phylogenetic tree, each pair of the Thai 
      isolates (01NP1 and 02KK1, 00CS1 and 01UD6, and 01CB1 and 01RB1) was identical 
      despite they were collected from different provinces. Therefore, there was no 
      geographic influence on the spreading of PRRSV in Thailand. Interestingly, 02CB12 
      (EU genotype) shared over 99% similarity of the ORF5 nucleotide sequence and 
      98.6% of amino acid identity with the European vaccine, Porcillis (AF378819). 
      However, modified live virus vaccines for PRRSV have not yet been used in the 
      swine population in Thailand. The results suggested that both US and EU genotypes 
      exist in Thailand, genetic variation does occur in both genotypes, and the 
      sources of the viruses appear to be from Canada and Northern Europe, 
      respectively. In addition, the spreading of PRRSV in Thailand might be due to 
      introducing infected replacement pigs or infected semen into the farm.
CI  - Copyright 2004 Elsiever B.V.
FAU - Thanawongnuwech, R
AU  - Thanawongnuwech R
AD  - Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn 
      University, Henri Dunant Road, Pathumwan, Bangkok 10330, Thailand. 
      roongroje.t@chula.ac.th
FAU - Amonsin, A
AU  - Amonsin A
FAU - Tatsanakit, A
AU  - Tatsanakit A
FAU - Damrongwatanapokin, S
AU  - Damrongwatanapokin S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cluster Analysis
MH  - Genetic Variation
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*genetics
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Alignment
MH  - Swine
MH  - Thailand/epidemiology
MH  - Viral Envelope Proteins/chemistry/genetics
EDAT- 2004/06/18 05:00
MHDA- 2004/09/08 05:00
CRDT- 2004/06/18 05:00
PHST- 2003/07/08 00:00 [received]
PHST- 2003/12/30 00:00 [revised]
PHST- 2004/03/14 00:00 [accepted]
PHST- 2004/06/18 05:00 [pubmed]
PHST- 2004/09/08 05:00 [medline]
PHST- 2004/06/18 05:00 [entrez]
AID - S0378113504000847 [pii]
AID - 10.1016/j.vetmic.2004.03.005 [doi]
PST - ppublish
SO  - Vet Microbiol. 2004 Jun 10;101(1):9-21. doi: 10.1016/j.vetmic.2004.03.005.