PMID- 15203929
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20191210
IS  - 1061-186X (Print)
IS  - 1026-7158 (Linking)
VI  - 11
IP  - 7
DP  - 2003 Aug
TI  - Cationic liposomes for gene transfection.
PG  - 407-14
AB  - Cationic liposomes spontaneously interact with the negatively charged DNA to form 
      a stable complex that promotes the gene transfer to cells. The mode of formation 
      and the size of cationic liposomes/DNA complexes were investigated using the 
      atomic force microscopy (AFM). Also the most important physical-chemical factors 
      involved in cationic liposome-mediated gene transfection, e.g. size and lipidic 
      composition, were evaluated through the transfection of complexes with different 
      liposomes/DNA molar ratio into three types of cultured cells. Cationic liposomes, 
      composed of a neutral lipid (phosphatidilcoline), a cationic lipid 
      dimethyldioctadecylammonium bromide (DDAB), a co-lipid 
      1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) and a phospholipid 
      derivative of polyethylene glycol (DSPE-mPEG) at different molar ratio, were 
      mixed with a plasmid pCMVbeta to form liposomes/DNA complexes. We have 
      demonstrated that the complexes were made by complicated structures in which the 
      liposomes tend to aggregate and the DNA is surrounded by lipidic material. In 
      vitro transfection efficiency by liposomes/plasmid pCMVbeta complexes was found 
      to depend on the kind of lipid associated in the liposomes and the liposomes/DNA 
      mixing ratio. The importance of associating DOPE in cationic liposomes was 
      confirmed; this co-lipid is able to improve the ability of cationic liposomes to 
      transfect cells but in addition, the AFM images and the EtBr fluorescence 
      experiments have suggested that this lipid can also play an important role to 
      facilitate the formation of stable liposomes, which efficaciously protect the DNA 
      by nuclease digestion.
FAU - Ruozi, Barbara
AU  - Ruozi B
AD  - Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, 
      Via Campi 183, 41100 Modena, Italy.
FAU - Forni, Flavio
AU  - Forni F
FAU - Battini, Renata
AU  - Battini R
FAU - Vandelli, Maria Angela
AU  - Vandelli MA
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Drug Target
JT  - Journal of drug targeting
JID - 9312476
RN  - 0 (Cations)
RN  - 0 (Drug Carriers)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Liposomes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cations
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cytomegalovirus/genetics
MH  - DNA/administration & dosage
MH  - Drug Carriers
MH  - Electrophoresis, Agar Gel
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Indicators and Reagents
MH  - Liposomes/chemical synthesis/*chemistry
MH  - Mice
MH  - Microscopy, Atomic Force
MH  - Particle Size
MH  - Plasmids/genetics
MH  - Transfection/*methods
EDAT- 2004/06/19 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/06/19 05:00
PHST- 2004/06/19 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/06/19 05:00 [entrez]
AID - 89TND13BDA94911N [pii]
AID - 10.1080/10611860310001655600 [doi]
PST - ppublish
SO  - J Drug Target. 2003 Aug;11(7):407-14. doi: 10.1080/10611860310001655600.