PMID- 15205373
OWN - NLM
STAT- MEDLINE
DCOM- 20041026
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 145
IP  - 10
DP  - 2004 Oct
TI  - The steroidogenic acute regulatory protein is expressed in steroidogenic cells of 
      the day-old brain.
PG  - 4775-80
AB  - Although recent research has focused on the fundamental role(s) of steroids 
      synthesized de novo in the brain on development, the mechanism by which 
      production of these neurosteroids is regulated remains unclear. Steroid 
      production in peripheral tissues is acutely regulated by the steroidogenic acute 
      regulatory (StAR) protein, which mediates the rate-limiting step in steroid 
      biosynthesis: the intramitochondrial delivery of cholesterol to cytochrome 
      P450scc for conversion to steroid. We recently demonstrated that StAR is present 
      in discrete cell types in the adult brain, suggesting that neurosteroid 
      production is mediated by StAR. Nevertheless, little is known regarding the 
      presence of StAR in the developing brain. In the present study, the presence of 
      StAR and for the first time, its homolog, the putative cholesterol transport 
      protein metastatic lymph node 64 (MLN64), were defined in the neonatal mouse 
      brain using immunocytochemical techniques. Both StAR and MLN64 were found to be 
      present in the brain with staining patterns characteristic to each protein, 
      indicating the authenticity of StAR and MLN64 immunoreactivity. Furthermore, we 
      found MLN64 to be expressed in the adult brain as well, apparently at higher 
      levels than StAR. Importantly, StAR protein is present in cells that also express 
      P450scc. These data suggest that, as with the adult, neurosteroid production 
      during development occurs through a StAR-mediated pathway.
FAU - King, Steven R
AU  - King SR
AD  - Room N730, Scott Department of Urology, Baylor College of Medicine, One Baylor 
      Plaza, Houston, Texas 77030-3498, USA. srking@bcm.tmc.edu
FAU - Ginsberg, Stephen D
AU  - Ginsberg SD
FAU - Ishii, Tomohiro
AU  - Ishii T
FAU - Smith, Roy G
AU  - Smith RG
FAU - Parker, Keith L
AU  - Parker KL
FAU - Lamb, Dolores J
AU  - Lamb DJ
LA  - eng
GR  - CA94520/CA/NCI NIH HHS/United States
GR  - DK54028/DK/NIDDK NIH HHS/United States
GR  - DK61548/DK/NIDDK NIH HHS/United States
GR  - NS43939/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040617
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (MLN64 protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*metabolism
MH  - Brain/*metabolism
MH  - Cholesterol Side-Chain Cleavage Enzyme/metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - Phosphoproteins/*metabolism
MH  - Tissue Distribution
EDAT- 2004/06/19 05:00
MHDA- 2004/10/27 09:00
CRDT- 2004/06/19 05:00
PHST- 2004/06/19 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/06/19 05:00 [entrez]
AID - en.2003-1740 [pii]
AID - 10.1210/en.2003-1740 [doi]
PST - ppublish
SO  - Endocrinology. 2004 Oct;145(10):4775-80. doi: 10.1210/en.2003-1740. Epub 2004 Jun 
      17.