PMID- 15205404
OWN - NLM
STAT- MEDLINE
DCOM- 20050309
LR  - 20071114
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 19
IP  - 9
DP  - 2004 Sep
TI  - Sperm selection for ICSI: shape properties do not predict the absence or presence 
      of numerical chromosomal aberrations.
PG  - 2052-9
AB  - BACKGROUND: We hypothesize that the potential relationship between abnormal sperm 
      morphology and increased frequency of numerical chromosomal aberrations is based 
      on two attributes of diminished sperm maturity: (i) cytoplasmic retention and 
      consequential sperm shape abnormalities; and (ii) meiotic errors caused by low 
      levels of the HspA2 chaperone, a component of the synaptonemal complex. Because 
      sperm morphology and aneuploidies were assessed in semen, but not in the same 
      spermatozoa, previous studies addressing this relationship were inconclusive. We 
      recently demonstrated that sperm shape is preserved following fluorescence in 
      situ hybridization (FISH). Thus, we examined the shape and chromosomal 
      aberrations in the same sperm. METHODS: We performed phase contrast microscopy 
      and FISH, using centromeric probes for chromosomes X, Y, 10, 11 and 17 in 15 men. 
      The fluorescence and respective phase contrast images were digitized using the 
      Metamorph program. We studied 1286 sperm (256 disomic, 130 diploid and 900 
      haploid sperm) by three criteria: head and tail dimensions, head shape and Kruger 
      strict morphology. Furthermore, in each analysis, we considered whether disomic 
      or diploid sperm may be distinguished from haploid sperm. RESULTS: There was an 
      overall, but not discriminative, relationship between abnormal sperm dimensions 
      or shape and increased frequencies of numerical chromosomal aberrations. However, 
      approximately 68 of the 256 disomic, and four of 130 diploid sperm showed head 
      and tail dimensions comparable with the most normal, lowest tertile of the 900 
      haploid spermatozoa. Considering all 1286 sperm, among those with the most 
      regular, symmetrical shape (n = 367), there were 63 and five with disomic and 
      diploid nuclei, respectively. In line with these findings, among the 256 disomic 
      sperm, 10% were Kruger normal. CONCLUSIONS: Sperm dimensions or shape are not 
      reliable attributes in selection of haploid sperm for ICSI.
FAU - Celik-Ozenci, Ciler
AU  - Celik-Ozenci C
AD  - The Sperm Physiology Laboratory, Department of Obstetrics and Gynecology, Yale 
      School of Medicine, New Haven, CT, USA.
FAU - Jakab, Attila
AU  - Jakab A
FAU - Kovacs, Tamas
AU  - Kovacs T
FAU - Catalanotti, Jillian
AU  - Catalanotti J
FAU - Demir, Ramazan
AU  - Demir R
FAU - Bray-Ward, Patricia
AU  - Bray-Ward P
FAU - Ward, David
AU  - Ward D
FAU - Huszar, Gabor
AU  - Huszar G
LA  - eng
GR  - HD-19505/HD/NICHD NIH HHS/United States
GR  - OH-04061/OH/NIOSH CDC HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040617
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Cell Shape/*genetics
MH  - *Chromosome Aberrations
MH  - Diploidy
MH  - Haploidy
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Phase-Contrast
MH  - Sperm Head/ultrastructure
MH  - *Sperm Injections, Intracytoplasmic
MH  - Sperm Tail/ultrastructure
MH  - Spermatozoa/*cytology/*physiology/ultrastructure
EDAT- 2004/06/19 05:00
MHDA- 2005/03/10 09:00
CRDT- 2004/06/19 05:00
PHST- 2004/06/19 05:00 [pubmed]
PHST- 2005/03/10 09:00 [medline]
PHST- 2004/06/19 05:00 [entrez]
AID - deh361 [pii]
AID - 10.1093/humrep/deh361 [doi]
PST - ppublish
SO  - Hum Reprod. 2004 Sep;19(9):2052-9. doi: 10.1093/humrep/deh361. Epub 2004 Jun 17.