PMID- 15205585
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20190917
IS  - 0957-5235 (Print)
IS  - 0957-5235 (Linking)
VI  - 15
IP  - 5
DP  - 2004 Jul
TI  - Effects of unfractionated heparin, low molecular weight heparin and r-hirudin on 
      leukocyte adhesion in ischemia/reperfusion.
PG  - 375-81
AB  - Activation of the coagulation cascade during myocardial ischemia and reperfusion 
      may contribute to the post-ischemic inflammatory response, mostly via generation 
      of thrombin. We assessed the effect of the anticoagulants unfractionated heparin 
      (UFH), low molecular weight heparin (LMWH) and r-hirudin on leukocyte adhesion 
      and emigration after ischemia and reperfusion in rats. The rat cremaster muscle 
      was prepared for intravital microscopy. One hundred and twenty minutes of 
      ischemia were followed by 90 min of reperfusion. Saline (control), UFH, LMWH or 
      r-hirudin were given 15 min prior to reperfusion and infused for the rest of the 
      observation period. Dosages per kilogram of body weight were (bolus, infusion): 
      saline, 3 ml, 3 ml/h; UFH, 400 IU, 100 IU/h; LMWH, 100 IU, 3 ml/h saline; or 
      r-hirudin, 0.3 mg, 0.15 mg/h. In collecting venules, rolling, adherent, and 
      extravasated leukocytes were counted from recordings of the intravital 
      microscopy. All three anticoagulants similarly attenuated post-ischemic 
      endothelial leukocyte adhesion. In contrast, emigration of leukocytes was only 
      attenuated by r-hirudin. The emigration efficiency of adherent leukocytes 
      (control, 1.21) was unchanged after UFH (1.74), and LMWH (1.51) but decreased 
      after r-hirudin treatment (0.12). The different efficacy of the three 
      anticoagulants in affecting emigration of adherent leukocytes suggests a specific 
      role for the direct thrombin inhibitor r-hirudin in attenuating the post-ischemic 
      inflammatory response. This effect may contribute to the benefits of direct 
      thrombin inhibitors seen in clinical studies after treatment for acute coronary 
      syndromes.
CI  - Copyright 2004 Lippincott Williams and Wilkins
FAU - Habazettl, Helmut
AU  - Habazettl H
AD  - Department of Physiology, Charite, Campus Benjamin Franklin, Berlin, Germany. 
      habazett@zedat.fu-berlin.de
FAU - Lindert, Jens
AU  - Lindert J
FAU - Baeter, Simonida
AU  - Baeter S
FAU - Neumann, Konrad
AU  - Neumann K
FAU - Kuppe, Hermann
AU  - Kuppe H
FAU - Kuebler, Wolfgang M
AU  - Kuebler WM
FAU - Pries, Axel R
AU  - Pries AR
FAU - Koster, Andreas
AU  - Koster A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Hirudins)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/*drug effects
MH  - Endothelium/pathology
MH  - Fibrinolytic Agents/*administration & dosage
MH  - Heparin/*administration & dosage
MH  - Heparin, Low-Molecular-Weight/*administration & dosage
MH  - Hirudins/*administration & dosage
MH  - Leukocytes/pathology
MH  - Male
MH  - Myocardial Ischemia/*drug therapy/*pathology/physiopathology
MH  - Myocardial Reperfusion Injury/*drug therapy/*pathology/physiopathology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2004/06/19 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/06/19 05:00
PHST- 2004/06/19 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/06/19 05:00 [entrez]
AID - 00001721-200407000-00002 [pii]
AID - 10.1097/01.mbc.0000114445.59147.43 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 2004 Jul;15(5):375-81. doi: 
      10.1097/01.mbc.0000114445.59147.43.