PMID- 15207092
OWN - NLM
STAT- MEDLINE
DCOM- 20050207
LR  - 20131121
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 20
IP  - 4
DP  - 2004 Jul
TI  - [Expression of monocyte chemoattractant protein-1 in glomerular endothelial cells 
      stimulated by high concentration of glucose].
PG  - 450-3
AB  - AIM: To study the expression of monocyte chemoattractant protein-1 (MCP-1) in 
      cultured human glomerular endothelial cells (HUGECs) stimulated by high 
      concentration of glucose. METHODS: The effect of high concentration of glucose on 
      the expression of MCP-1mRNA by HUGECs was detected by in situ hybridization and 
      cell ELISA. The monocyte migration induced by the conditioned media of cultured 
      HUGECs was assayed by a modified Boyden Chamber micropore filtration membrane 
      method. Effects of anti-MCP-1 antibody on monocyte migration was detected as 
      well. RESULTS: The MCP-1 mRNA was only weakly expressed in HUGECs cultured under 
      the condition of low concentration of glucose. Following a stimulation of high 
      concentration of glucose (25 mmol/L), MCP-1 mRNA expression was upregulated from 
      the 8th hour and reached the maximum at the 16th hour. Conditioned medium of 
      cultured HUGECs stimulated with high concentration of glucose had marked 
      chemotaxis for monocytes, which was inhibited by anti-MCP-1 antibody. CONCLUSION: 
      HUGEC stimulated by high glucose may highly express MCP-1, and produce chemotatic 
      factors for monocytes.
FAU - Ding, Han-lu
AU  - Ding HL
AD  - Department of Nephrology, Daping Hospital, Third Military Medical University, 
      Chongqing 400042, China. dhanlu@eyou.com
FAU - Zhang, Jian-guo
AU  - Zhang JG
FAU - Zhu, Miao-zhen
AU  - Zhu MZ
FAU - Luo, Xiang-dong
AU  - Luo XD
FAU - Liang, Guang-ping
AU  - Liang GP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics/immunology
MH  - Culture Media, Conditioned/pharmacology
MH  - Endothelial Cells/*metabolism
MH  - Glucose/administration & dosage/*pharmacology
MH  - Humans
MH  - Kidney Glomerulus/cytology/*metabolism
MH  - Leukemia, Myeloid/pathology
MH  - Monocytes/pathology/physiology
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2004/06/23 05:00
MHDA- 2005/02/08 09:00
CRDT- 2004/06/23 05:00
PHST- 2004/06/23 05:00 [pubmed]
PHST- 2005/02/08 09:00 [medline]
PHST- 2004/06/23 05:00 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Jul;20(4):450-3.