PMID- 15207250
OWN - NLM
STAT- MEDLINE
DCOM- 20050124
LR  - 20171116
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 27
IP  - 1
DP  - 2004 Jul 7
TI  - High level expression, activation, and antagonism of CC chemokine receptors CCR2 
      and CCR3 in Chinese hamster ovary cells.
PG  - 38-46
AB  - The specificity of leukocyte trafficking in inflammation is controlled by the 
      interactions of chemokines with chemokine receptors. Reliable structure-function 
      studies of chemokine-receptor interactions would benefit from cell lines that 
      express consistent high levels of chemokine receptors. We describe herein two new 
      Chinese hamster ovary (CHO) cell lines in which the genes for chemokine receptors 
      CCR2 and CCR3 have been incorporated into identical positions in the host genome. 
      CCR2 is the primary receptor for the chemokine monocyte chemoattractant protein-1 
      (MCP-1) whereas CCR3 is the primary receptor for the chemokines eotaxin-1, 
      eotaxin-2 and eotaxin-3. Both receptors are expressed at >5,000,000 copies per 
      cell, substantially higher levels than in previous cell lines, and both are 
      competent for binding and activation by the cognate chemokines for these 
      receptors. Using these cell lines we confirm that eotaxin-1 and eotaxin-3 can act 
      as an agonist and an antagonist, respectively, of CCR2. In addition, we show that 
      eotaxin-2 is an antagonist of CCR2 and MCP-1 is an agonist of CCR3. Comparison of 
      the chemokine sequences reveals several positions that are identical in MCP-1 and 
      eotaxin-1 but different in eotaxin-2 and eotaxin-3, suggesting that these amino 
      acids play a role in CCR2 activation.
FAU - Parody, Todd R
AU  - Parody TR
AD  - Department of Chemistry, Indiana University, Bloomington, IN 47405-0001, USA.
FAU - Stone, Martin J
AU  - Stone MJ
LA  - eng
GR  - GM 55055/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (CCL11 protein, human)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL26 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (CCR3 protein, human)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL26)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, CCR3)
RN  - 0 (Receptors, Chemokine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding, Competitive
MH  - CHO Cells
MH  - Calcium/metabolism
MH  - Chemokine CCL11
MH  - Chemokine CCL2/pharmacology/physiology
MH  - Chemokine CCL26
MH  - Chemokines/*pharmacology/physiology
MH  - Chemokines, CC/pharmacology/physiology
MH  - Cricetinae
MH  - Cricetulus
MH  - Molecular Sequence Data
MH  - Radioligand Assay
MH  - Receptors, CCR2
MH  - Receptors, CCR3
MH  - Receptors, Chemokine/*agonists/*antagonists & inhibitors/metabolism
MH  - Sequence Alignment
MH  - Structure-Activity Relationship
EDAT- 2004/06/23 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/06/23 05:00
PHST- 2003/10/18 00:00 [received]
PHST- 2004/03/16 00:00 [revised]
PHST- 2004/03/30 00:00 [accepted]
PHST- 2004/06/23 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/06/23 05:00 [entrez]
AID - S1043466604000985 [pii]
AID - 10.1016/j.cyto.2004.03.013 [doi]
PST - ppublish
SO  - Cytokine. 2004 Jul 7;27(1):38-46. doi: 10.1016/j.cyto.2004.03.013.