PMID- 15207754
OWN - NLM
STAT- MEDLINE
DCOM- 20050819
LR  - 20161124
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 35
IP  - 1
DP  - 2004 Jul
TI  - Abnormal bone collagen morphology and decreased bone strength in growth 
      hormone-deficient rats.
PG  - 178-85
AB  - Patients with growth hormone deficiency (GHD) have an increased risk of bone 
      fractures. In these patients, the well-described decrease in bone mineral density 
      (BMD) and content (BMC) may, however, not alone explain the increase in fracture 
      rate. Accordingly, the aim of this study was to evaluate collagen morphology and 
      bone mineralisation in cortical bone as well as bone strength in GHD rats to try 
      to clarify the explanation for the increased fracture rate. The Dw-4 rat was used 
      as a model for GHD. This strain of rats has an autosomal recessive disorder, 
      reducing GH synthesis to approximately 10% and growth rate to approximately 
      40-50% when compared to normal control rats. Five male Dw-4 rats were examined at 
      age 12 weeks and five healthy Lewis rats served as age-matched controls. The 
      animals were examined for (1) bone mineral status by dual energy X-ray 
      absorptometry (DXA) and ash weight/bone volume, (2) biomechanical properties, (3) 
      serum insulin-like growth factor I (IGF-I) and IGF binding protein 3 (IGFBP-3), 
      and (4) collagen morphology of cortical bone from the right femurs was examined 
      by scanning and transmission electron microscopy. A significant decrease was 
      found in serum IGF-I, IGFBP-3 and biomechanical properties in GHD rats compared 
      to controls (P < 0.009). While DXA-derived BMD was decreased, no significant 
      difference was found in ash weight/bone volume. Electron microscopy showed a 
      significant decrease in the number and a significant increase in the diameter of 
      collagen microfibrils in GHD rats as compared to their controls (P < 0.009). In 
      conclusion, we report for the first time that collagen morphology in bone is 
      markedly altered in rats with isolated GHD. Whether similar conditions are 
      present in GHD patients need further investigations. The changes described, 
      however, may provide a co-explanation for the increased fracture rate in GHD.
FAU - Lange, Martin
AU  - Lange M
AD  - Department of Endocrinology and Nephrology, Rigshospitalet, Copenhagen, Denmark. 
      mlange@rh.dk
FAU - Qvortrup, Klaus
AU  - Qvortrup K
FAU - Svendsen, Ole Lander
AU  - Svendsen OL
FAU - Flyvbjerg, Allan
AU  - Flyvbjerg A
FAU - Nowak, Jette
AU  - Nowak J
FAU - Petersen, Michael M
AU  - Petersen MM
FAU - OLgaard, Klaus
AU  - OLgaard K
FAU - Feldt-Rasmussen, Ulla
AU  - Feldt-Rasmussen U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Fibrillar Collagens)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Absorptiometry, Photon
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Bone Density
MH  - Bone and Bones/diagnostic imaging/*ultrastructure
MH  - Collagen/*ultrastructure
MH  - Extracellular Matrix/ultrastructure
MH  - Female
MH  - Fibrillar Collagens/ultrastructure
MH  - Growth Hormone/*deficiency
MH  - Insulin-Like Growth Factor Binding Protein 3/blood
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Rats
EDAT- 2004/06/23 05:00
MHDA- 2005/08/20 09:00
CRDT- 2004/06/23 05:00
PHST- 2003/11/06 00:00 [received]
PHST- 2004/02/11 00:00 [revised]
PHST- 2004/02/17 00:00 [accepted]
PHST- 2004/06/23 05:00 [pubmed]
PHST- 2005/08/20 09:00 [medline]
PHST- 2004/06/23 05:00 [entrez]
AID - S8756328204000808 [pii]
AID - 10.1016/j.bone.2004.02.025 [doi]
PST - ppublish
SO  - Bone. 2004 Jul;35(1):178-85. doi: 10.1016/j.bone.2004.02.025.