PMID- 15209388
OWN - NLM
STAT- MEDLINE
DCOM- 20041019
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 51
IP  - 3
DP  - 2004 Mar
TI  - Human amniotic fluid cells are able to produce IL-6 and IL-8.
PG  - 198-203
AB  - PROBLEM: In order to investigate the role of amniotic fluid cells (AFC) in the 
      establishment of feto-maternal immune relationship, we evaluated their phenotype 
      and capacity to produce cytokines. METHODS OF STUDY: CK 7-8, human leukocyte 
      antigen (HLA)-I, HLA-DR, HLA-G, CD1d, CD34, CD45, CD14 surface antigens 
      expression and the intracellular production of IL-4, IL-6, IL-8, IL-10, IL-12, 
      interferon-gamma and tumor necrosis factor-1 were studied in cultured AFC and in 
      eight samples immediately after amniocentesis using flow cytometry. IL-6 and IL-8 
      were detected by ELISA in all amniotic fluids and in all culture supernatants. 
      Moreover, IL-6 and IL-8 mRNA were tested in nine samples. RESULTS: Cultured AFC 
      express HLA-I, HLA-G and CK 7-8 and are able to produce IL-6 and IL-8, confirmed 
      by presence of their mRNA. We quantified IL-6 and IL-8 levels in all amniotic 
      fluids and in all supernatants. CONCLUSION: Surface antigen expression of AFC are 
      not specific of immune cells, but AFC are able to produce cytokines and we can 
      postulate that they may participate in mechanisms involved in normal as well as 
      pathological pregnancy.
FAU - Mazzucchelli, Iolanda
AU  - Mazzucchelli I
AD  - Laboratori Sperimentali, Area Trapiantologica, IRCCS Policlinico San Matteo, 
      Pavia, Italia.
FAU - Avanzini, Maria Antonietta
AU  - Avanzini MA
FAU - Ciardelli, Laura
AU  - Ciardelli L
FAU - Pagani, Sara
AU  - Pagani S
FAU - Greco, Rossella
AU  - Greco R
FAU - Belloni, Cesare
AU  - Belloni C
FAU - Castellazzi, AnnaMaria
AU  - Castellazzi A
FAU - Marconi, Massimo
AU  - Marconi M
FAU - Rondini, Giorgio
AU  - Rondini G
FAU - Polatti, Franco
AU  - Polatti F
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Amniotic Fluid/*cytology/immunology/*metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Flow Cytometry
MH  - HLA Antigens/genetics/immunology/metabolism
MH  - Humans
MH  - Interleukin-6/genetics/immunology/*metabolism
MH  - Interleukin-8/genetics/immunology/*metabolism
MH  - Middle Aged
MH  - RNA, Messenger/analysis/genetics
EDAT- 2004/06/24 05:00
MHDA- 2004/10/20 09:00
CRDT- 2004/06/24 05:00
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2004/10/20 09:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - 10.1111/j.1600-0897.2004.00137.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2004 Mar;51(3):198-203. doi: 
      10.1111/j.1600-0897.2004.00137.x.