PMID- 15210758
OWN - NLM
STAT- MEDLINE
DCOM- 20040817
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 173
IP  - 1
DP  - 2004 Jul 1
TI  - Immature dendritic cells (DCs) use chemokines and intercellular adhesion molecule 
      (ICAM)-1, but not DC-specific ICAM-3-grabbing nonintegrin, to stimulate CD4+ T 
      cells in the absence of exogenous antigen.
PG  - 50-60
AB  - Dendritic cells (DCs) possess a number of unique features that distinguish them 
      from other APCs. One such feature is their ability to trigger Ag-independent 
      responses in T cells. Previous studies have focused on mature DCs, but the 
      prevalence of this phenomenon in the resting-state immature DCs has never been 
      considered. In this study, we show that, in the absence of Ag, human immature DCs 
      trigger multiple responses in autologous primary CD4+ T cells, namely, increased 
      motility, small Ca2+ transients, and up-regulation of CD69. These responses are 
      particularly marked in CD4+ memory T cells. By using several experimental 
      approaches, we found that DC-specific ICAM-3-grabbing nonintegrin plays no role 
      in the induction of T cell responses, whereas ICAM-1/LFA-1 interactions are 
      required. In addition, DC-produced chemokines contribute to the Ag-independent T 
      cell stimulatory ability of DCs, because pertussis toxin-treated T cells exhibit 
      diminished responses to immature DCs. More particularly, CCL17 and CCL22, which 
      are constitutively produced by immature DCs, mediate both T cell polarization and 
      attraction. Thus, immature DCs owe part of their outstanding Ag-independent T 
      cell stimulatory ability to chemokines and ICAM-1, but not DC-specific 
      ICAM-3-grabbing nonintegrin.
FAU - Real, Eliana
AU  - Real E
AD  - Departement de Biologie Cellulaire, Institut Cochin, Institut National de la 
      Sante et de la Recherche Medicale, Unite 567, Centre National de la Recherche 
      Scientifique (CNRS), Unite Mixte de Recherche (UMR) 8104, Institut Pasteur, 
      Paris, France.
FAU - Kaiser, Andrew
AU  - Kaiser A
FAU - Raposo, Graca
AU  - Raposo G
FAU - Amara, Ali
AU  - Amara A
FAU - Nardin, Alessandra
AU  - Nardin A
FAU - Trautmann, Alain
AU  - Trautmann A
FAU - Donnadieu, Emmanuel
AU  - Donnadieu E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CCL17 protein, human)
RN  - 0 (CCL22 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokine CCL22)
RN  - 0 (Chemokines, CC)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology/*physiology
MH  - Cell Adhesion Molecules/*physiology
MH  - Cell Movement
MH  - Chemokine CCL17
MH  - Chemokine CCL22
MH  - Chemokines, CC/*physiology
MH  - Dendritic Cells/*physiology
MH  - Humans
MH  - Immunologic Memory
MH  - Intercellular Adhesion Molecule-1/*physiology
MH  - Lectins, C-Type/*physiology
MH  - Receptors, Cell Surface/*physiology
EDAT- 2004/06/24 05:00
MHDA- 2004/08/18 05:00
CRDT- 2004/06/24 05:00
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2004/08/18 05:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - 10.4049/jimmunol.173.1.50 [doi]
PST - ppublish
SO  - J Immunol. 2004 Jul 1;173(1):50-60. doi: 10.4049/jimmunol.173.1.50.