PMID- 15212708
OWN - NLM
STAT- MEDLINE
DCOM- 20050329
LR  - 20181130
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 24
IP  - 6
DP  - 2004 Jun
TI  - Synergism between interleukin-11 and bone morphogenetic protein-2 in the healing 
      of segmental bone defects in a rabbit model.
PG  - 343-9
AB  - Recombinant human interleukin-11 (rHuIL-11) and recombinant human bone 
      morphogenetic protein-2 (rHuBMP-2) have been shown to act synergistically in the 
      induction of osteoblast differentiation. To determine whether these two proteins 
      can be used clinically in fracture healing and reconstructive surgery, we 
      investigated whether rHuIL-11 and rHuBMP-2 act synergistically to heal segmental 
      bone defects in a rabbit model. A 1.5-cm segmental defect was created in the 
      right ulnar diaphysis of 20 Japanese white rabbits. Polylactic-co-glycolic acid 
      (PLGA)-coated gelatin sponges (PGS) permeated with rHuBMP-2 (n = 8), rHuIL-11 
      plus rHuBMP-2 (n = 8), or rHuIL-11 (n = 4) were implanted into the bone defects. 
      Radiographs were scored by two independent observers for bone formation and union 
      rates after 2, 3, 4, and 8 weeks. Bone formation was higher in rabbits implanted 
      with rHuBMP-2 plus rHuIL-11 than in those implanted with rHuBMP-2 alone, reaching 
      statistical significance after 4 weeks. At early time points, the union rate in 
      rabbits implanted with rHuBMP-2 plus rHuIL-11 was higher than in rabbits 
      implanted with rHuBMP-2. At 2, 4, and 8 weeks, new bone volume was significantly 
      higher in rabbits administered rHuIL-11 plus rHuBMP-2 than in those given 
      rHuBMP-2 alone. In contrast, mechanical testing after 8 weeks showed that bone 
      strength in the two groups of rabbits was equivalent. These findings show that 
      rHuIL-11 and rHuBMP-2 act synergistically to accelerate bone formation without 
      affecting bone strength. Treatment with a combination of rHuIL-11 and rHuBMP-2 
      may thus be of great benefit in fracture healing and for patients undergoing 
      reconstructive surgery.
FAU - Suga, Kazutaka
AU  - Suga K
AD  - Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi 
      Pharmaceutical Co. Ltd., Tsukuba, Ibaraki, Japan. suga.kazutaka@yamanouchi.co.jp
FAU - Saitoh, Minori
AU  - Saitoh M
FAU - Kokubo, Satoshi
AU  - Kokubo S
FAU - Nozaki, Kazutoshi
AU  - Nozaki K
FAU - Fukushima, Shinji
AU  - Fukushima S
FAU - Yasuda, Shuhei
AU  - Yasuda S
FAU - Sasamata, Masao
AU  - Sasamata M
FAU - Miyata, Keiji
AU  - Miyata K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (BMP2 protein, human)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Drug Carriers)
RN  - 0 (Interleukin-11)
RN  - 0 (Polymers)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (recombinant human bone morphogenetic protein-2)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/metabolism
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Bone Regeneration/*drug effects/physiology
MH  - Drug Carriers/metabolism
MH  - *Drug Synergism
MH  - *Fracture Healing
MH  - Humans
MH  - Interleukin-11/*pharmacology
MH  - Lactic Acid/metabolism
MH  - Male
MH  - Polyglycolic Acid/metabolism
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/metabolism
MH  - Rabbits
MH  - Radiography
MH  - Recombinant Proteins
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Ulna/diagnostic imaging/pathology
EDAT- 2004/06/24 05:00
MHDA- 2005/03/30 09:00
CRDT- 2004/06/24 05:00
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2005/03/30 09:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - 10.1089/107999004323142204 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2004 Jun;24(6):343-9. doi: 10.1089/107999004323142204.