PMID- 15212811
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20141120
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 200
IP  - 2-3
DP  - 2004 Aug 5
TI  - Response of human cord blood cells to styrene exposure: evaluation of its effects 
      on apoptosis and gene expression by genomic technology.
PG  - 145-57
AB  - Styrene is one of the most important monomers produced worldwide, and it finds 
      major use in the production of polystyrene, acrylonitrile-butadiene-styrene 
      resins and unsaturated polystyrene resins. Epidemiological studies on styrene 
      showed that the malignancies observed most frequently in humans after exposure 
      are related to the lymphatic and haemopoietic system. IARC classified styrene a 
      possible carcinogenic to humans (Group 2B). In this study, we evaluated the 
      effect of styrene on gene expression profiles of human cord blood cells, as well 
      as its activity on the apoptosis and bcl-2 related protein expression. Data 
      demonstrated that, after 24 and 48 h of exposure, styrene (800 microM) induced an 
      increase in the necrosis of mononuclear cord blood cells, whereas it did not 
      cause any increase in the apoptotic process. Western blot analysis revealed a 
      modified expression of Bax, BCl-2, c-Jun, c-Fos and Raf-1 proteins in the human 
      cord blood cells after direct exposure to styrene, whereas p53 expression did not 
      change. Furthermore, Macroarray analysis showed that styrene changed cord blood 
      gene expression, inducing up-regulation of monocyte chemotactic protein 1 
      (MCP-1), and down-regulation of CC chemokine receptor type 1 (CCR-1) and SLP-76 
      tyrosine-phosphoprotein.
FAU - Diodovich, Cristina
AU  - Diodovich C
AD  - Laboratory of Hematotoxicology, ECVAM Institute for Health and Consumer 
      Protection, JRC, Ispra, Italy. cristina.diodovich@jrc.it
FAU - Bianchi, Marco Giorgio
AU  - Bianchi MG
FAU - Bowe, Gerard
AU  - Bowe G
FAU - Acquati, Francesco
AU  - Acquati F
FAU - Taramelli, Roberto
AU  - Taramelli R
FAU - Parent-Massin, Dominique
AU  - Parent-Massin D
FAU - Gribaldo, Laura
AU  - Gribaldo L
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (DNA Primers)
RN  - 44LJ2U959V (Styrene)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Cell Division/drug effects
MH  - DNA Primers
MH  - Drug Industry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erythroid Precursor Cells
MH  - Fetal Blood/cytology/*drug effects
MH  - Flow Cytometry
MH  - Gene Expression/*drug effects
MH  - *Genetic Engineering
MH  - Humans
MH  - In Vitro Techniques
MH  - Necrosis
MH  - Oligonucleotide Array Sequence Analysis
MH  - Protein Biosynthesis
MH  - RNA/biosynthesis/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Styrene/*toxicity
EDAT- 2004/06/24 05:00
MHDA- 2004/08/04 05:00
CRDT- 2004/06/24 05:00
PHST- 2004/01/22 00:00 [received]
PHST- 2004/03/19 00:00 [revised]
PHST- 2004/03/19 00:00 [accepted]
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - S0300483X04001817 [pii]
AID - 10.1016/j.tox.2004.03.021 [doi]
PST - ppublish
SO  - Toxicology. 2004 Aug 5;200(2-3):145-57. doi: 10.1016/j.tox.2004.03.021.