PMID- 15213154
OWN - NLM
STAT- MEDLINE
DCOM- 20040806
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 72
IP  - 7
DP  - 2004 Jul
TI  - Synthetic fragments of Vibrio cholerae O1 Inaba O-specific polysaccharide bound 
      to a protein carrier are immunogenic in mice but do not induce protective 
      antibodies.
PG  - 4090-101
AB  - Development of Vibrio cholerae lipopolysaccharide (LPS) as a cholera vaccine 
      immunogen is justified by the correlation of vibriocidal anti-LPS response with 
      immunity. Two V. cholerae O1 LPS serotypes, Inaba and Ogawa, are associated with 
      endemic and pandemic cholera. Both serotypes induce protective antibody following 
      infection or vaccination. Structurally, the LPSs that define the serotypes are 
      identical except for the terminal perosamine moiety, which has a methoxyl group 
      at position 2 in Ogawa but a hydroxyl group in Inaba. The terminal sugar of the 
      Ogawa LPS is a protective B-cell epitope. We chemically synthesized the terminal 
      hexasaccharides of V. cholerae serotype Ogawa, which comprises in part the 
      O-specific polysaccharide component of the native LPS, and coupled the 
      oligosaccharide at different molar ratios to bovine serum albumin (BSA). Our 
      initial studies with Ogawa immunogens showed that the conjugates induced 
      protective antibody. We hypothesized that antibodies specific for the terminal 
      sugar of Inaba LPS would also be protective. Neoglycoconjugates were prepared 
      from synthetic Inaba oligosaccharides (disaccharide, tetrasaccharide, and 
      hexasaccharide) and BSA at different levels of substitution. BALB/c mice 
      responded to the Inaba carbohydrate (CHO)-BSA conjugates with levels of serum 
      antibodies of comparable magnitude to those of mice immunized with Ogawa CHO-BSA 
      conjugates, but the Inaba-specific antibodies (immunoglobulin M [IgM] and IgG1) 
      were neither vibriocidal nor protective in the infant mouse cholera model. We 
      hypothesize that the anti-Inaba antibodies induced by the Inaba CHO-BSA 
      conjugates have enough affinity to be screened via enzyme-linked immunosorbent 
      assay but not enough to be protective in vivo.
FAU - Meeks, Michael D
AU  - Meeks MD
AD  - Department of Microbiology and Immunology, Dartmouth Medical School, 630 W. 
      Borwell Bldg., Lebanon, NH 03756, USA.
FAU - Saksena, Rina
AU  - Saksena R
FAU - Ma, Xingquan
AU  - Ma X
FAU - Wade, Terri K
AU  - Wade TK
FAU - Taylor, Ronald K
AU  - Taylor RK
FAU - Kovac, Pavol
AU  - Kovac P
FAU - Wade, William F
AU  - Wade WF
LA  - eng
GR  - R01 AI025096/AI/NIAID NIH HHS/United States
GR  - R37 AI025096/AI/NIAID NIH HHS/United States
GR  - AI 25096/AI/NIAID NIH HHS/United States
GR  - AI 47373/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies)
RN  - 0 (O Antigens)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Antibodies/*immunology
MH  - Female
MH  - Mice
MH  - O Antigens/*immunology/metabolism
MH  - Serum Albumin, Bovine/metabolism
MH  - Vibrio cholerae O1/*immunology
PMC - PMC427411
EDAT- 2004/06/24 05:00
MHDA- 2004/08/07 05:00
PMCR- 2004/07/01
CRDT- 2004/06/24 05:00
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2004/08/07 05:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - 72/7/4090 [pii]
AID - 0182-04 [pii]
AID - 10.1128/IAI.72.7.4090-4101.2004 [doi]
PST - ppublish
SO  - Infect Immun. 2004 Jul;72(7):4090-101. doi: 10.1128/IAI.72.7.4090-4101.2004.