PMID- 15213406
OWN - NLM
STAT- MEDLINE
DCOM- 20050308
LR  - 20081015
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 60
IP  - Pt 7
DP  - 2004 Jul
TI  - Expression, crystallization and preliminary X-ray characterization of the 
      Drosophila transcription factor Doublesex.
PG  - 1328-30
AB  - Doublesex (DSX) is a transcription factor in Drosophila melanogaster that 
      regulates somatic sexual differentiation. Homologues have been found in diverse 
      metazoans, suggesting a conserved role in development. Here, the expression, 
      purification and crystallization of a novel 63-amino-acid dimerization domain and 
      selenomethionine analogue are described. Native crystals belong to space group 
      P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. 
      Selenomethionine-containing crystals provide anomalous dispersion for phasing.
FAU - Bayrer, James
AU  - Bayrer J
AD  - Case Western Reserve University, Cleveland, Ohio, USA.
FAU - Wan, Zhuli
AU  - Wan Z
FAU - Li, Biaoru
AU  - Li B
FAU - Weiss, Michael A
AU  - Weiss MA
LA  - eng
GR  - T32GM07250/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040622
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DSX protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - DNA-Binding Proteins/*chemistry/genetics/isolation & purification
MH  - Drosophila Proteins/*chemistry/genetics/isolation & purification
MH  - Drosophila melanogaster/*chemistry/genetics
MH  - Gene Expression
MH  - Transcription Factors/*chemistry/genetics/isolation & purification
EDAT- 2004/06/24 05:00
MHDA- 2005/03/09 09:00
CRDT- 2004/06/24 05:00
PHST- 2004/05/04 00:00 [received]
PHST- 2004/05/19 00:00 [accepted]
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2005/03/09 09:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - S0907444904012223 [pii]
AID - 10.1107/S0907444904012223 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1328-30. doi: 
      10.1107/S0907444904012223. Epub 2004 Jun 22.