PMID- 15215630
OWN - NLM
STAT- MEDLINE
DCOM- 20050124
LR  - 20190823
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 48
IP  - 5
DP  - 2004
TI  - Identification of beta-tubulin isoform V as an autoantigen in allergic rhinitis 
      by a proteomic approach.
PG  - 427-34
AB  - Autoantibodies to IgE and beta2-adrenergic receptor have been reported in 
      patients with allergic rhinitis. To investigate whether autoimmunity in allergic 
      rhinitis is directed to such limited molecules or directed to a wide range of 
      self proteins, we here attempted to survey autoantigens/autoantibodies 
      comprehensively, using proteomics. Specifically, we separated proteins extracted 
      from peripheral blood mononuclear cells by 2-dimensional electrophoresis and then 
      detected autoantigens by subsequent western blotting with sera from patients with 
      allergic rhinitis. As a result, we detected multiple autoantigens, some of which 
      were further identified by mass fingerprinting. Next, we confirmed antigenicity 
      of one of the identified autoantigens, beta-tubulin isoform V (beta-tubV), using 
      a recombinant protein and then measured prevalence of the anti-beta-tubV 
      autoantibodies. As a result, 52% of the tested patients with allergic rhinitis 
      were found to possess anti-beta-tubV autoantibodies. Our study indicates that 
      autoimmunity is a common phenomena and beta-tubV is one of the major autoantigens 
      in allergic rhinitis.
FAU - Nakamura, Manabu
AU  - Nakamura M
AD  - Department of Bioregulation, Institute of Medical Science, St. Marianna 
      University School of Medicine, Kawasaki, Kanagawa, Japan.
FAU - Tsutsumi, Kouichiro
AU  - Tsutsumi K
FAU - Ooka, Seido
AU  - Ooka S
FAU - Sekine, Taichi
AU  - Sekine T
FAU - Koizuka, Izumi
AU  - Koizuka I
FAU - Nishioka, Kusuki
AU  - Nishioka K
FAU - Kato, Tomohiro
AU  - Kato T
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Adrenergic, beta-2)
RN  - 0 (Tubulin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Autoantibodies/blood/immunology
MH  - Autoantigens/*immunology/isolation & purification
MH  - Child
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/immunology
MH  - Leukocytes, Mononuclear/chemistry
MH  - Male
MH  - Mass Spectrometry
MH  - Middle Aged
MH  - Protein Isoforms/immunology/isolation & purification
MH  - Proteins/immunology/isolation & purification
MH  - *Proteomics
MH  - Receptors, Adrenergic, beta-2/immunology
MH  - Rhinitis, Allergic, Perennial/*immunology
MH  - Tubulin/*immunology/isolation & purification
EDAT- 2004/06/25 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/06/25 05:00
PHST- 2004/06/25 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/06/25 05:00 [entrez]
AID - 10.1111/j.1348-0421.2004.tb03532.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2004;48(5):427-34. doi: 10.1111/j.1348-0421.2004.tb03532.x.