PMID- 1521935
OWN - NLM
STAT- MEDLINE
DCOM- 19921009
LR  - 20190907
IS  - 0192-0561 (Print)
IS  - 0192-0561 (Linking)
VI  - 14
IP  - 4
DP  - 1992 May
TI  - Popliteal lymph node enlargement induced by procainamide.
PG  - 681-6
AB  - The ability of procainamide (PA) to induce primary local popliteal lymph node 
      (PLN) reactions has been investigated. We employed an in vitro drug-metabolizing 
      system, based on the hypothesis that the negative result for PA in a PLN assay 
      (PLNA) was due to insufficient metabolizing activity at the reaction site. PA was 
      incubated previously in vitro with the S-9 mixture derived from rat liver. The 
      reactants were ultrafiltered in order to eliminate high molecular-weight 
      molecules, and then the low molecular-weight fraction was subcutaneously (s.c.) 
      injected into the hind footpad of mice. PLN reactions were assessed by weighing 
      the popliteal lymph node of the injected side. The reactants of more than 5 mg of 
      PA and S-9 mixture induced PLN enlargement in C3H/He mice 8 days after injection. 
      BALB/c and C57BL/6 mice were also susceptible to PLN reaction in response to the 
      reactants of PA (10 mg) and S-9 mixture. PLN reactions to PA were induced 2 days 
      after the injection and sustained until 18 days. Contact of PA with the S-9 
      mixture for 30 min at 37 degrees C was sufficient to induce PLN enlargement. 
      However, contact for 24 h reduced the peak reaction. On the other hand, PA which 
      had not been incubated with the S-9 mixture and acetylprocainamide (acetylPA) 
      gave little or no reaction. Local PNA has not been considered to be suitable for 
      the detection of drugs with the potential to induce immune disorders in cases 
      where a metabolite contributes to the adverse reaction. However, the employment 
      of an in vitro drug-metabolizing system may overcome that defect.
FAU - Katsutani, N
AU  - Katsutani N
AD  - Department of Drug Safety Research, Eisai Co., Ltd, Gifu, Japan.
FAU - Shionoya, H
AU  - Shionoya H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Immunopharmacol
JT  - International journal of immunopharmacology
JID - 7904799
RN  - L39WTC366D (Procainamide)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - In Vitro Techniques
MH  - Lymph Nodes/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Procainamide/*pharmacology
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 0192-0561(92)90131-4 [pii]
AID - 10.1016/0192-0561(92)90131-4 [doi]
PST - ppublish
SO  - Int J Immunopharmacol. 1992 May;14(4):681-6. doi: 10.1016/0192-0561(92)90131-4.