PMID- 15219379
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20131121
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 6
DP  - 2004 Jun
TI  - Determination of the peptide binding motif and high-affinity ligands for HLA-DQ4 
      using synthetic peptide libraries.
PG  - 594-601
AB  - Juvenile idiopathic arthritis (JIA) is considered to be an autoimmune disease. 
      Various human leukocyte antigen (HLA) associations for different subgroups of 
      this heterogeneous disease have been found. For early-onset pauciarticular 
      arthritis (now oligoarthritic JIA), a strong association with the HLA class II 
      haplotype DQA1*0401/DQB1*0402 (DQ4) has been described. We determined the 
      peptide-binding specificities of this HLA-DQ molecule by screening a synthetic 
      acetylated nonapeptide amide library with one defined and eight random sequence 
      positions. A characteristic binding motif could be deduced. By use of these data, 
      we designed defined specific nonapeptides and identified high-affinity ligands 
      binding to HLA-DQ4. The peptide binding motif of HLA-DQ4 is very similar to the 
      motif of HLA-DQ7, also associated with oligoarthritic JIA. It is, however, 
      different from binding motifs of neutral or protective HLA-DQ molecules. Our 
      results further support the idea of differential peptide presentation in the 
      pathogenesis of oligoarthritic JIA.
FAU - Volz, Thomas
AU  - Volz T
AD  - University Children's Hospital, Tuebingen, Germany.
FAU - Schwarz, Gerold
AU  - Schwarz G
FAU - Fleckenstein, Burkhard
AU  - Fleckenstein B
FAU - Schepp, Carsten P
AU  - Schepp CP
FAU - Haug, Markus
AU  - Haug M
FAU - Roth, Johannes
AU  - Roth J
FAU - Wiesmuller, Karl-Heinz
AU  - Wiesmuller KH
FAU - Dannecker, Gunther E
AU  - Dannecker GE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ4 antigen)
RN  - 0 (HLA-DQ7 antigen)
RN  - 0 (Ligands)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Motifs/immunology
MH  - Amino Acid Sequence
MH  - Arthritis, Juvenile/*immunology/pathology
MH  - Cell Line, Transformed
MH  - HLA-DQ Antigens/*immunology/metabolism
MH  - Humans
MH  - Ligands
MH  - Molecular Sequence Data
MH  - Peptide Library
MH  - Peptides/chemical synthesis/*immunology/metabolism
MH  - Protein Binding/immunology
EDAT- 2004/06/29 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/06/29 05:00
PHST- 2003/11/13 00:00 [received]
PHST- 2004/02/20 00:00 [revised]
PHST- 2004/02/24 00:00 [accepted]
PHST- 2004/06/29 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/06/29 05:00 [entrez]
AID - S0198885904000710 [pii]
AID - 10.1016/j.humimm.2004.02.027 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Jun;65(6):594-601. doi: 10.1016/j.humimm.2004.02.027.