PMID- 15219387
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20040628
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 6
DP  - 2004 Jun
TI  - Identification of two pseudogenes with sequence homology to human and gorilla MHC 
      class IA genes: ancestral haplotype in the Filipino population.
PG  - 665-73
AB  - While characterizing exons 2 and 3 of the class I human leukocyte antigen (HLA)-A 
      locus in human lymphocytes, two similar but unexpected PCR products were detected 
      in six samples of Filipino ethnicity. A nucleotide sequence analysis of the two 
      amplicons, tentatively named HLA-COQ and HLA-DEL, rendered them as two novel and 
      seemingly related sequences, both with homology to the gorilla and human major 
      histocompatibility complex (MHC) A locus. Exon 2 is similar to the published 
      human pseudogenes HLA-BEL, HLA-Y, and to primate MHC Gogo-A*0501, differing by 2 
      bp from HLA-BEL, and HLA-Y, and by 4 bp from Gogo-A*0501. Exon 3 is most similar 
      to HLA-A*2902 and A*310102, differing by 7 bp from A*2902, and by 8 bp from 
      A*31012. Genomic sequence comparison of exons 1 to 8 indicates that their closest 
      published match is to the Gogo-A*0501. Complete typing at the HLA-A, -B, -C, 
      DRB1, and DRB5 loci for the six samples yielded the reoccurring types: 
      HLA-A*3401, -B*1521/1525, -Cw*0403, -DRB1*150201, and DRB5*010101. Thus far, 
      HLA-COQ and HLA-DEL have been detected only in Filipino samples containing these 
      HLA types. The HLA-COQ gene is nonfunctional based on a stop codon located in 
      exon 4. HLA-DEL is also a nonfunctional gene because of the dual cytosine 
      insertion in exon 4, with a reading frame shift generating a stop codon 
      downstream. Parsimony analysis of the two pseudogenes with 31 other primate A 
      locus coding regions resulted in a phylogenetic tree that segregated the two 
      pseudogenes with the Gogo-A*0501, suggesting that HLA-COQ, HLA-DEL, and 
      Gogo-A*0501 evolved from a common ancestral allele.
FAU - Coquillard, G
AU  - Coquillard G
AD  - Children's Memorial Hospital, Chicago, IL, USA. g-coquillard@northwestern.edu
FAU - Lau, M
AU  - Lau M
FAU - Kletzel, M
AU  - Kletzel M
FAU - Rodriguez-Marino, S G
AU  - Rodriguez-Marino SG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - *Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Exons/*genetics/immunology
MH  - Frameshift Mutation/genetics/immunology
MH  - Gorilla gorilla/*genetics/immunology
MH  - Histocompatibility Antigens Class I/*genetics/immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Philippines
MH  - *Phylogeny
MH  - Pseudogenes/*genetics/immunology
EDAT- 2004/06/29 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/06/29 05:00
PHST- 2004/01/16 00:00 [received]
PHST- 2004/02/12 00:00 [accepted]
PHST- 2004/06/29 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/06/29 05:00 [entrez]
AID - S0198885904000618 [pii]
AID - 10.1016/j.humimm.2004.02.025 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Jun;65(6):665-73. doi: 10.1016/j.humimm.2004.02.025.