PMID- 15221185
OWN - NLM
STAT- MEDLINE
DCOM- 20050815
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 379
IP  - 7-8
DP  - 2004 Aug
TI  - Eliminating absorbing interference using the H-point standard addition method: 
      case of Griess assay in the presence of interferent heme enzymes such as NOS.
PG  - 1055-61
AB  - Standard calibration methods used to determine trace analytes usually yield 
      significant deviations from the actual analyte value in the presence of 
      interferents in the assay media. These deviations become of particular concern 
      when the concentration of the analyte is low, and when the results are used to 
      draw mechanistic or kinetic conclusions, for instance in enzyme 
      structure-function studies. In these circumstances, the H-point standard addition 
      method (HPSAM) provides superior precision and accuracy. This method is developed 
      here for the case of the spectrophotometric Griess assay used to determine 
      nitrite in various enzymology investigations, such as nitrite determination in 
      studies of nitrite reductases (NiR), or when determining nitrite as a breakdown 
      product of nitric oxide synthesized by NOS enzymes. The results obtained by HPSAM 
      are contrasted with those of the traditional calibration method.
FAU - Indika, Perera N
AU  - Indika PN
AD  - Chemistry Department, Cleveland State University, SR 397, Cleveland, OH 44115, 
      USA.
FAU - Bayachou, Mekki
AU  - Bayachou M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040622
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Ethylenediamines)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Griess reagent)
RN  - 0 (Myoglobin)
RN  - 0 (Nitrites)
RN  - 0 (Sulfanilamides)
RN  - 42VZT0U6YR (Heme)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Calibration
MH  - Ethylenediamines
MH  - Free Radical Scavengers/*chemistry
MH  - Heme/*chemistry
MH  - Horses
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Mice
MH  - Myoglobin/chemistry/standards
MH  - Nitric Oxide Synthase/*chemistry/standards
MH  - Nitric Oxide Synthase Type II
MH  - Nitrites/analysis
MH  - Sensitivity and Specificity
MH  - Spectrophotometry/methods
MH  - Sulfanilamides
EDAT- 2004/06/29 05:00
MHDA- 2005/08/16 09:00
CRDT- 2004/06/29 05:00
PHST- 2004/01/05 00:00 [received]
PHST- 2004/04/26 00:00 [revised]
PHST- 2004/04/30 00:00 [accepted]
PHST- 2004/06/29 05:00 [pubmed]
PHST- 2005/08/16 09:00 [medline]
PHST- 2004/06/29 05:00 [entrez]
AID - 10.1007/s00216-004-2674-2 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2004 Aug;379(7-8):1055-61. doi: 10.1007/s00216-004-2674-2. 
      Epub 2004 Jun 22.