PMID- 1522232
OWN - NLM
STAT- MEDLINE
DCOM- 19921013
LR  - 20220227
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 90
IP  - 3
DP  - 1992 Sep
TI  - Enhanced production of monocyte chemoattractant protein-1 in rheumatoid 
      arthritis.
PG  - 772-9
AB  - Cells within the synovial tissue may recruit mononuclear phagocytes into the 
      synovial fluid and tissues of arthritic patients. We investigated the production 
      of the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1) using 
      sera, synovial fluid, synovial tissue, as well as macrophages and fibroblasts 
      isolated from synovial tissues from 80 arthritic patients. MCP-1 levels were 
      significantly higher (P less than 0.05) in synovial fluid from RA patients (mean 
      25.5 +/- 8.1 ng/ml [SE]) compared to synovial fluid from osteoarthritis (OA) 
      patients (0.92 +/- 0.08), or from patients with other arthritides (2.9 +/- 1.5). 
      MCP-1 levels in RA sera (8.44 +/- 2.33) were significantly greater than MCP-1 in 
      normal sera (0.16 +/- 0.06). The quantities of RA synovial fluid IL-8, which is 
      chemotactic for neutrophils and lymphocytes, and MCP-1 were strongly positively 
      correlated (P less than 0.05). To examine the cellular source of MCP-1, RA 
      synovial tissue macrophages and fibroblasts were isolated. Synovial tissue 
      fibroblasts did not express MCP-1 mRNA, but could be induced to produce MCP-1 by 
      stimulation with either IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), or 
      LPS. In contrast, unlike normal peripheral blood monocytes or alveolar 
      macrophages, RA synovial tissue macrophages constitutively expressed MCP-1 mRNA 
      and antigen. Immunohistochemical analysis of synovial tissue showed that a 
      significantly greater percentage of RA macrophages (50 +/- 8%) as compared to 
      either OA macrophages (5 +/- 2) or normal macrophages (1 +/- 0.3) reacted with 
      anti-MCP-1 antibodies. In addition, the synovial lining layer reacted with MCP-1 
      in both RA and OA synovial tissues. In contrast, only a minority of synovial 
      fibroblasts (18 +/- 8%) from RA synovium were positive for immunolocalization of 
      MCP-1. These results suggest that synovial production of MCP-1 may play an 
      important role in the recruitment of mononuclear phagocytes during inflammation 
      associated with RA and that synovial tissue macrophages are the dominant source 
      of this cytokine.
FAU - Koch, A E
AU  - Koch AE
AD  - Department of Medicine, Northwestern University Medical School, Chicago, Illinois 
      60611.
FAU - Kunkel, S L
AU  - Kunkel SL
FAU - Harlow, L A
AU  - Harlow LA
FAU - Johnson, B
AU  - Johnson B
FAU - Evanoff, H L
AU  - Evanoff HL
FAU - Haines, G K
AU  - Haines GK
FAU - Burdick, M D
AU  - Burdick MD
FAU - Pope, R M
AU  - Pope RM
FAU - Strieter, R M
AU  - Strieter RM
LA  - eng
GR  - AR30692/AR/NIAMS NIH HHS/United States
GR  - AR41492/AR/NIAMS NIH HHS/United States
GR  - HL-02401/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Arthritis, Rheumatoid/*metabolism
MH  - Base Sequence
MH  - Chemokine CCL2
MH  - Chemotactic Factors/analysis/*biosynthesis/genetics
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-8/analysis
MH  - Macrophages/metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Synovial Fluid/metabolism
PMC - PMC329929
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
PMCR- 1992/09/01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
PHST- 1992/09/01 00:00 [pmc-release]
AID - 10.1172/JCI115950 [doi]
PST - ppublish
SO  - J Clin Invest. 1992 Sep;90(3):772-9. doi: 10.1172/JCI115950.