PMID- 15224155
OWN - NLM
STAT- MEDLINE
DCOM- 20060721
LR  - 20131121
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 56
IP  - 3
DP  - 2004 Jun 25
TI  - [Inhibitory effect of caffeine on GABA-activated current in acutely isolated rat 
      dorsal root ganglion neurons].
PG  - 384-8
AB  - By means of whole-cell patch clamp technique, the modulatory effect of caffeine 
      on GABA-activated currents (I(GABA)) was investigated in acutely isolated rat 
      dorsal root ganglion (DRG) neurons. The majority of the neurons examined 
      (113/116) were sensitive to GABA (1~1000 micromol/L). GABA activated a 
      concentration-dependent inward current, which manifested obvious desensitization. 
      In 58 out of 108 neurons, caffeine induced a small inward current, while in 
      others no detectable current was observed. After the neurons were treated with 
      caffeine (0.1~100 micromol/L) prior to the application of GABA (100 micromol/L) 
      for 30 s, GABA-activated inward currents were obviously inhibited. Caffeine 
      shifted the GABA dose-response curve downward and decreased the maximum response 
      to 57% without changing K(d) value. These results indicate that the inhibitory 
      effect is non-competitive. The pretreatment with caffeine (10 micromol/L) 
      inhibited I(GABA) which was potentiated by diazepam (1 micromol/L). Intracellular 
      application of H-8 almost completely abolished the inhibitory effect of caffeine 
      on I(GABA). Because GABA can induce primary afferent depolarization (PAD), our 
      results suggest that caffeine may be able to antagonize the effect of presynaptic 
      inhibition of GABA in primary afferent.
FAU - Li, Shao
AU  - Li S
AD  - Department of Physiology and Research Center of Brain Disorders, Dalian Medical 
      University, Dalian 116027, China.
FAU - An, Jie
AU  - An J
FAU - Sun, Chang-Kai
AU  - Sun CK
FAU - Li, Zhi-Wang
AU  - Li ZW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (GABA Antagonists)
RN  - 0 (Receptors, GABA-A)
RN  - 3G6A5W338E (Caffeine)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Caffeine/*pharmacology
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Electrophysiology
MH  - GABA Antagonists/pharmacology
MH  - Ganglia, Spinal/cytology/*physiology
MH  - Neurons/cytology/physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA-A/physiology
MH  - gamma-Aminobutyric Acid/*physiology
EDAT- 2004/06/30 05:00
MHDA- 2006/07/22 09:00
CRDT- 2004/06/30 05:00
PHST- 2004/06/30 05:00 [pubmed]
PHST- 2006/07/22 09:00 [medline]
PHST- 2004/06/30 05:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2004 Jun 25;56(3):384-8.