PMID- 15228091
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 260
IP  - 1-2
DP  - 2004 May
TI  - Modulation of intracellular calcium concentrations and T cell activation by 
      prickly pear polyphenols.
PG  - 103-10
AB  - Opuntia ficus indica (prickly pear) polyphenolic compounds (OFPC) triggered an 
      increase in [Ca2+]i in human Jurkat T-cell lines. Furthermore, OFPC-induced rise 
      in [Ca2+]i was significantly curtailed in calcium-free buffer (0% Ca2+) as 
      compared to that in 100% Ca2+ medium. Preincubation of cells with tyrphostin A9, 
      an inhibitor of Ca2+ release-activated Ca2+ (CRAC) channels, significantly 
      diminished the OFPC-induced sustained response on the increases in [Ca2+]i. 
      Lanthanum and nifedipine, the respective inhibitors of voltage-dependent and 
      L-type calcium channels, failed to curtail significantly the OFPC-induced calcium 
      response. As OFPC still stimulated increases in [Ca2+]i in 0% Ca2+ medium, the 
      role of intracellular calcium was investigated. Hence, addition of thapsigargin 
      (TG), an inhibitor of Ca2+-ATPase of the endoplasmic reticulum (ER), during the 
      OFPC-induced peak response exerted an additive effect, indicating that the 
      mechanism of action of these two agents are different. Furthermore, U73122, an 
      inhibitor of IP3 production, completely abolished increases in [Ca2+]i, induced 
      by OFPC, suggesting that these polyphenols induce the production of IP3 that 
      recruits calcium from ER pool. Polyphenolic compounds do act extracellularly as 
      addition of fatty acid-free bovine serum albumin (BSA) significantly diminished 
      the rise in [Ca2+]i evoked by the formers. OFPC also induced plasma membrane 
      hyperpolarisation which was reversed by addition of BSA. OFPC were found to 
      curtail the expression of IL-2 mRNA and T-cell blastogenesis. Together these 
      results suggest that OFPC induce increases in [Ca2+]i via ER pool and opening of 
      CRAC channels, and exert immunosuppressive effects in Jurkat T-cells.
FAU - Aires, Virginie
AU  - Aires V
AD  - UPRES Lipides and Nutrition, Universite de Bourgogne, Faculte des Sciences de la 
      vie 6, Dijon, France.
FAU - Adote, Sylvie
AU  - Adote S
FAU - Hichami, Aziz
AU  - Hichami A
FAU - Moutairou, Kabirou
AU  - Moutairou K
FAU - Boustani, Es-Saddik E
AU  - Boustani ES
FAU - Khan, Naim A
AU  - Khan NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Flavonoids)
RN  - 0 (Interleukin-2)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tyrphostins)
RN  - 10537-47-0 (tyrphostin A9)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism/pharmacology
MH  - Calcium Signaling/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Flavonoids/*pharmacology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Interleukin-2/genetics
MH  - Jurkat Cells
MH  - Lymphocyte Activation/drug effects
MH  - Membrane Potentials/drug effects
MH  - Opuntia/*chemistry
MH  - Phenols/*pharmacology
MH  - Plant Extracts/pharmacology
MH  - Polyphenols
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*drug effects/immunology/metabolism
MH  - Thapsigargin/pharmacology
MH  - Tyrphostins/pharmacology
EDAT- 2004/07/02 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/07/02 05:00
PHST- 2004/07/02 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/07/02 05:00 [entrez]
AID - 10.1023/b:mcbi.0000026061.57326.28 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2004 May;260(1-2):103-10. doi: 
      10.1023/b:mcbi.0000026061.57326.28.