PMID- 15228654
OWN - NLM
STAT- MEDLINE
DCOM- 20041028
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 12
IP  - 3
DP  - 2004 Jun
TI  - [Analysis and identification of variant Ph chromosome translocation in patients 
      with chronic myelogenous leukemia by conventional cytogenetics and fluorescence 
      in situ hybridization].
PG  - 298-303
AB  - The objective of this study was to explore the cytogenetic profiles of variant Ph 
      chromosome translocations (VT) in patients with chronic myelogenous leukemia 
      (CML) and to assess the applications of fluorescence in situ hybridization (FISH) 
      technique for analysis of CML patients with variant translocation by using dual 
      color-single fusion signal (DC-SF) and dual color-dual fusion signal (DC-DF) 
      probe. 42 CML patients with VT were studied by conventional cytogenetic analysis 
      (CCA). Among them, nine and eleven cases were analyzed by DC-SF-FISH and 
      DC-DF-FISH, respectively. The results showed that 42 out of 643 (6.5%) CML cases 
      received CCA were found to have VT, which were composed of 18 cases of simple VT, 
      23 of complex VT and one of masked VT. The VT involved all over the chromosomes 
      but No. 4 and 6. Four patterns of them appeared recurrent because each occurred 
      in at least two cases. VT with additional chromosomal aberrations were shown in 
      35.7% of patients with VT (15/42). 19 of 20 patients who received FISH detection 
      were positive for bcr/abl fusion. DC-DF-FISH analysis revealed absence of abl/bcr 
      fusion signal in all patients but one (8.8%) with abl/bcr positive cells. 
      However, it was not an implication of gene loss but the translocation led to part 
      of bcr retaining on der (9q34) and other part of bcr translocating to involve 
      another chromosome. It was unable to observe variant signal features by 
      DC-SF-FISH analysis. In conclusion, variant Ph translocations in CML involved 
      almost all chromosomes in a varying frequencies and ways except chromosomes 9 and 
      22, and some of them showed recurrent aberrations. FISH provides accurate 
      molecular diagnosis for CML with VT, while DC-DF-FISH facilitates the assessment 
      of variant signals.
FAU - Liu, Xu-Ping
AU  - Liu XP
AD  - Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Tianjin 300020, China.
FAU - Liu, Shi-He
AU  - Liu SH
FAU - Li, Cheng-Wen
AU  - Li CW
FAU - Bo, Li-Jin
AU  - Bo LJ
FAU - Qin, Shuang
AU  - Qin S
FAU - Dai, Yun
AU  - Dai Y
FAU - Qian, Lin-Sheng
AU  - Qian LS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
EDAT- 2004/07/02 05:00
MHDA- 2004/10/29 09:00
CRDT- 2004/07/02 05:00
PHST- 2004/07/02 05:00 [pubmed]
PHST- 2004/10/29 09:00 [medline]
PHST- 2004/07/02 05:00 [entrez]
AID - 1009-2137(2004)03-0298-06 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2004 Jun;12(3):298-303.