PMID- 15229120
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20071114
IS  - 0002-9262 (Print)
IS  - 0002-9262 (Linking)
VI  - 160
IP  - 1
DP  - 2004 Jul 1
TI  - Optimizing buccal cell DNA yields in mothers and infants for human leukocyte 
      antigen genotyping.
PG  - 77-84
AB  - Buccal cells provide a convenient source of DNA for epidemiologic studies. 
      Mouthwash rinses yield a higher quality and quantity of DNA than cytobrushes but 
      are not practical for collection from infants. Although cytobrushes yield 
      sufficient DNA for most genotyping analyses, human leukocyte antigen (HLA) 
      analysis can require 1,000-fold more DNA. In Iowa City, Iowa, in 2002, the 
      authors tested two cytobrush collection methods to optimize total DNA yield and 
      purity for HLA genotyping in mothers and infants: 1) brushing the left and right 
      inner cheeks (standard method) and 2) brushing the upper and lower "gutters", 
      that is, the space between the gums and the inner lips/cheeks along the front and 
      sides of the mouth (test method). Storage and mailing experiments were performed 
      to define conditions for optimizing DNA yield and purity. Mothers' gutter samples 
      yielded significantly higher total amounts of DNA (mean yield = 15.0 micro g/two 
      brushes) than cheek samples (mean yield = 7.6 micro g/two brushes) (paired t 
      test: p < 0.001), while DNA yields from cheek and gutter collections from infants 
      were equivalent. Cytobrushes stored and/or mailed in paper envelopes yielded 
      significantly more and higher-purity DNA than brushes in plastic bags or tubes. 
      Cytobrush sampling of the mouth's gutter areas can enhance DNA yield in mothers 
      but not in young infants. DNA yields can be further optimized by controlling 
      mailing and storage conditions.
CI  - Copyright 2004 Johns Hopkins Bloomberg School of Public Health
FAU - Saftlas, Audrey F
AU  - Saftlas AF
AD  - Department of Epidemiology, University of Iowa College of Public Health, Iowa 
      City, IA 52242, USA. audrey-saftlas@uiowa.edu
FAU - Waldschmidt, Marianella
AU  - Waldschmidt M
FAU - Logsden-Sackett, Nyla
AU  - Logsden-Sackett N
FAU - Triche, Elizabeth
AU  - Triche E
FAU - Field, Elizabeth
AU  - Field E
LA  - eng
GR  - R01-HD-325790/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Epidemiol
JT  - American journal of epidemiology
JID - 7910653
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cheek
MH  - DNA/genetics/*isolation & purification
MH  - Female
MH  - *Genotype
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Infant
MH  - Iowa
MH  - Mothers
MH  - *Mouth Mucosa
MH  - Pilot Projects
MH  - Specimen Handling/*methods
EDAT- 2004/07/02 05:00
MHDA- 2004/08/04 05:00
CRDT- 2004/07/02 05:00
PHST- 2004/07/02 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2004/07/02 05:00 [entrez]
AID - 160/1/77 [pii]
AID - 10.1093/aje/kwh171 [doi]
PST - ppublish
SO  - Am J Epidemiol. 2004 Jul 1;160(1):77-84. doi: 10.1093/aje/kwh171.