PMID- 15231384
OWN - NLM
STAT- MEDLINE
DCOM- 20040817
LR  - 20181130
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 325
IP  - 1
DP  - 2004 Jul 20
TI  - Towards a molecular identification and classification system of 
      lepidopteran-specific baculoviruses.
PG  - 36-47
AB  - Virus genomics provides novel approaches for virus identification and 
      classification. Based on the comparative analyses of sequenced 
      lepidopteran-specific baculovirus genomes, degenerate oligonucleotides were 
      developed that allow the specific amplification of several regions of the genome 
      using polymerase chain reaction (PCR) followed by DNA sequencing. The DNA 
      sequences within the coding regions of three highly conserved genes, namely 
      polyhedrin/granulin (polh/gran), late expression factor 8 (lef-8), and late 
      expression factor 9 (lef-9), were targeted for amplification. The 
      oligonucleotides were tested on viral DNAs isolated from historical field 
      samples, and amplification products were generated from 12 isolated 
      nucleopolyhedrovirus (NPV) and 8 granulovirus (GV) DNAs. The PCR products were 
      cloned or directly sequenced, and phylogenetic trees were inferred from 
      individual and combined data sets of these three genes and compared to a 
      phylogeny, which includes 22 baculoviruses using a combined data set of 30 core 
      genes. This method allows a fast and reliable detection and identification of 
      lepidopteran-specific NPVs and GVs. Furthermore, a strong correlation of the base 
      composition of these three genome areas with that of the complete virus genome 
      was observed and used to predict the base composition of uncharacterized 
      baculovirus genomes. These analyses suggested that GVs have a significantly 
      higher AT content than NPVs.
FAU - Lange, Martin
AU  - Lange M
AD  - Laboratory for Biotechnological Crop Protection, Department of Phytopathology, 
      Agricultural Service Center Palatinate, 67435 Neustadt an der Weinstrasse, 
      Germany.
FAU - Wang, Hualin
AU  - Wang H
FAU - Zhihong, Hu
AU  - Zhihong H
FAU - Jehle, Johannes A
AU  - Jehle JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (late expression factor 8, Baculovirus)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Animals
MH  - Baculoviridae/*classification/genetics
MH  - Genome, Viral
MH  - Lepidoptera/*virology
MH  - Occlusion Body Matrix Proteins
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Viral Proteins/genetics
MH  - Viral Structural Proteins
EDAT- 2004/07/03 05:00
MHDA- 2004/08/18 05:00
CRDT- 2004/07/03 05:00
PHST- 2004/03/04 00:00 [received]
PHST- 2004/04/01 00:00 [revised]
PHST- 2004/04/15 00:00 [accepted]
PHST- 2004/07/03 05:00 [pubmed]
PHST- 2004/08/18 05:00 [medline]
PHST- 2004/07/03 05:00 [entrez]
AID - S0042682204002867 [pii]
AID - 10.1016/j.virol.2004.04.023 [doi]
PST - ppublish
SO  - Virology. 2004 Jul 20;325(1):36-47. doi: 10.1016/j.virol.2004.04.023.