PMID- 15231570
OWN - NLM
STAT- MEDLINE
DCOM- 20041214
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 9
DP  - 2004 Nov 1
TI  - HIV-1-infected dendritic cells up-regulate cell surface markers but fail to 
      produce IL-12 p70 in response to CD40 ligand stimulation.
PG  - 2810-7
AB  - Dendritic cells (DCs) are antigen-presenting cells with the capacity to prime 
      naive T cells for efficient cellular responses against pathogens such as HIV-1. 
      DCs are also susceptible to HIV-1 infection, which may impair their ability to 
      induce immunity. Here, we examined the ability of HIV-1-infected, in 
      vitro-derived DCs to respond to CD40 ligand (CD40L) stimulation with the aim to 
      study events during early HIV-1 infection. HIV-1(BaL)-infected p24(+) DCs were 
      detected after only 3 days of exposure to highly concentrated virus. We show that 
      HIV-1-infected DCs up-regulated costimulatory molecules, but were skewed in their 
      production of effector cytokines in response to CD40L stimulation. CD40L 
      stimulation induced significant secretion of tumor necrosis factor alpha 
      (TNFalpha) and interleukin 12 (IL-12) p70 from both HIV-1-exposed and unexposed 
      DCs. Intracellular stainings of HIV-1-exposed DCs revealed that TNFalpha could be 
      detected in both the p24(-) and p24(+) DCs, but IL-12 p70 could be found only in 
      the p24(-) DCs. Thus, although p24(+) DCs showed a mature phenotype similar to 
      p24(-) DCs after CD40L stimulation, they appeared to have an impaired cytokine 
      profile. These observations suggest that HIV-1 infection disables DC function, a 
      phenomenon that may be relevant for optimal induction of HIV-1-specific immune 
      responses.
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
AD  - Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, 
      F59 Karolinska University Hospital Huddinge, S-141 86 Stockholm, Sweden. 
      anna.smed.sorensen@medhs.ki.se
FAU - Lore, Karin
AU  - Lore K
FAU - Walther-Jallow, Lilian
AU  - Walther-Jallow L
FAU - Andersson, Jan
AU  - Andersson J
FAU - Spetz, Anna-Lena
AU  - Spetz AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040701
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, Surface)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, CD1/analysis
MH  - Antigens, Surface/*analysis
MH  - B7-2 Antigen
MH  - CD40 Ligand/immunology/*pharmacology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/immunology/metabolism/*virology
MH  - HIV Core Protein p24/analysis
MH  - HIV Infections/*immunology
MH  - HIV-1
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-12/biosynthesis/*metabolism
MH  - Membrane Glycoproteins/analysis
MH  - Protein Subunits/biosynthesis/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
EDAT- 2004/07/03 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/07/03 05:00
PHST- 2004/07/03 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/07/03 05:00 [entrez]
AID - S0006-4971(20)55951-X [pii]
AID - 10.1182/blood-2003-07-2314 [doi]
PST - ppublish
SO  - Blood. 2004 Nov 1;104(9):2810-7. doi: 10.1182/blood-2003-07-2314. Epub 2004 Jul 
      1.