PMID- 15233768
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20220129
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 20
IP  - 2
DP  - 2004 Jul
TI  - Intraocular delivery of BDNF following visual cortex lesion upregulates cytosolic 
      branched chain aminotransferase (BCATc) in the rat dorsal lateral geniculate 
      nucleus.
PG  - 580-6
AB  - Visual cortex ablation in newborn rats determines the almost complete 
      degeneration of neurons in the dorsal lateral geniculate nucleus (dLGN), as a 
      consequence of the axotomy of the geniculo-cortical fibres. Death of dLGN neurons 
      is massive and rapid, and occurs by apoptosis. We recently showed that exogenous 
      administration of the neurotrophin brain-derived neurotrophic factor (BDNF) in 
      the eye prevents the degeneration of dLGN neurons occurring after visual cortex 
      lesion in newborn rats. To elucidate the molecular mechanisms of BDNF-mediated 
      neuroprotection, we sought to identify novel genes regulated by BDNF in the rat 
      dLGN after visual cortex lesion. By using mRNA fingerprinting, we isolated a cDNA 
      fragment upregulated in the dLGN of lesioned rats treated with BDNF. This cDNA 
      fragment shared 100% homology with the rat cytosolic branched chain 
      aminotransferase (BCATc), a key enzyme of glutamate metabolism. Quantitative 
      reverse transcription-polymerase chain reaction and in situ hybridization 
      confirmed that BCATc mRNA is markedly overexpressed by exogenous supply of BDNF 
      to axotomized dLGNs. Immunohistochemical analysis showed that upregulation of 
      BCATc in the dLGN of lesioned rats treated with BDNF takes place in astrocytes. 
      These results suggest that modulation of glutamate metabolism by astrocytes might 
      play an important role in BDNF-mediated survival of axotomized dLGN neurons.
FAU - Madeddu, Federico
AU  - Madeddu F
AD  - Istituto di Neuroscienze del C.N.R., Pisa, Italy.
FAU - Naska, Sibel
AU  - Naska S
FAU - Menna, Elisabetta
AU  - Menna E
FAU - Chiellini, Chiara
AU  - Chiellini C
FAU - Sweatt, Andrew J
AU  - Sweatt AJ
FAU - Hutson, Susan M
AU  - Hutson SM
FAU - Benzi, Luca
AU  - Benzi L
FAU - Maffei, Margherita
AU  - Maffei M
FAU - Maffei, Lamberto
AU  - Maffei L
FAU - Bozzi, Yuri
AU  - Bozzi Y
LA  - eng
GR  - TCP99016/TI_/Telethon/Italy
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.42 (branched-chain-amino-acid transaminase)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Northern/methods
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Count
MH  - DNA Fingerprinting/methods
MH  - Geniculate Bodies/*drug effects/enzymology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glutamate Decarboxylase/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Neuroglia/drug effects/metabolism
MH  - Neurons/drug effects/metabolism
MH  - Phosphopyruvate Hydratase/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Long-Evans
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Transaminases/genetics/*metabolism
MH  - Visual Cortex/drug effects/*pathology
EDAT- 2004/07/06 05:00
MHDA- 2004/10/01 05:00
CRDT- 2004/07/06 05:00
PHST- 2004/07/06 05:00 [pubmed]
PHST- 2004/10/01 05:00 [medline]
PHST- 2004/07/06 05:00 [entrez]
AID - EJN3482 [pii]
AID - 10.1111/j.1460-9568.2004.03482.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2004 Jul;20(2):580-6. doi: 10.1111/j.1460-9568.2004.03482.x.