PMID- 15234523
OWN - NLM
STAT- MEDLINE
DCOM- 20040917
LR  - 20131121
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 58
IP  - 2
DP  - 2004 Aug
TI  - The development of a DNA microarray-based assay for the characterization of 
      commercially formulated microbial products.
PG  - 251-62
AB  - Commercially formulated bioproducts containing a complex consortia of bacteria as 
      an active ingredient pose a significant challenge for regulatory agencies and 
      companies seeking to assess the safety and efficacy of these bioproducts. The 
      main challenge stems from how to characterize the bacterial composition of these 
      products, for which there is presently a lack of suitable methods. A prototype 
      DNA microarray composed of oligonucleotide probes for functional genes, virulence 
      factors, and taxonomic genes for a number of bacterial species was developed to 
      examine the utility of microarray technology as a molecular tool for 
      characterizing consortia bioproducts. The genomic DNA from four different 
      products was extracted by two methods and examined with the microarray prototype 
      and by denaturing gradient gel electrophoresis (DGGE). Although the identity of 
      the consortial species remains unknown, the microarray assay provided unique and 
      reproducible hybridization patterns for all four products, and agreed with the 
      fingerprints generated by DGGE. The ability to differentiate between a variety of 
      consortia products demonstrates that DNA microarrays have the potential to be a 
      powerful tool in monitoring complex microbial communities.
FAU - Dubois, J W
AU  - Dubois JW
AD  - Environmental Sector, Biotechnology Research Institute, NRC, 6100 Royalmount 
      Avenue, Montreal, Quebec, Canada H4P 2R2.
FAU - Hill, S
AU  - Hill S
FAU - England, L S
AU  - England LS
FAU - Edge, T
AU  - Edge T
FAU - Masson, L
AU  - Masson L
FAU - Trevors, J T
AU  - Trevors JT
FAU - Brousseau, R
AU  - Brousseau R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Biological Products)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacteriological Techniques
MH  - Base Sequence
MH  - Biological Products/*analysis/*genetics
MH  - DNA Fingerprinting
MH  - DNA Probes/genetics
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Genes, Bacterial
MH  - Molecular Sequence Data
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Polymerase Chain Reaction
EDAT- 2004/07/06 05:00
MHDA- 2004/09/21 05:00
CRDT- 2004/07/06 05:00
PHST- 2004/01/19 00:00 [received]
PHST- 2004/04/05 00:00 [revised]
PHST- 2004/04/08 00:00 [accepted]
PHST- 2004/07/06 05:00 [pubmed]
PHST- 2004/09/21 05:00 [medline]
PHST- 2004/07/06 05:00 [entrez]
AID - S0167701204001125 [pii]
AID - 10.1016/j.mimet.2004.04.011 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2004 Aug;58(2):251-62. doi: 10.1016/j.mimet.2004.04.011.