PMID- 15236931
OWN - NLM
STAT- MEDLINE
DCOM- 20050602
LR  - 20061115
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 28
IP  - 10
DP  - 2004 Aug
TI  - Anaphylatoxin-like molecules generated during complement activation induce a 
      dramatic enhancement of particle uptake in rainbow trout phagocytes.
PG  - 1005-21
AB  - Here we have identified a serum fraction containing approximately 8-kDa molecules 
      with an unexpected capacity to greatly enhance particle uptake in trout head 
      kidney leukocytes (HKLs). This approximately 8-kDa particle-uptake enhancing 
      fraction (PUEF-8) was purified from complement-activated serum by gel filtration 
      chromatography. Mass spectrometric analysis and reactivity of anti-trout C3-1 and 
      C4 antibodies, indicated the presence of C3a, C4a and C5a molecules in PUEF-8. 
      Using a newly developed flow cytometric assay that measures the capacity of cells 
      to ingest fluorescent beads, we showed that PUEF-8 induced a striking enhancement 
      (344+/-50% higher than the PBS control value) in the number of HKLs ingesting 
      three or more beads. In contrast, the effect of PUEF-8 on peripheral blood 
      leukocytes (PBLs) was almost negligible. Interestingly, PUEF-8 acted as a strong 
      chemoattractant for both HKLs and PBLs. These findings suggest a novel role for 
      the anaphylatoxins generated during complement activation in teleost fish.
FAU - Li, J
AU  - Li J
AD  - Department of Pathobiology, School of Veterinary Medicine, University of 
      Pennsylvania, 413 Rosenthal, 3800 Spruce St., Philadelphia, PA 19104, USA.
FAU - Peters, R
AU  - Peters R
FAU - Lapatra, S
AU  - Lapatra S
FAU - Vazzana, M
AU  - Vazzana M
FAU - Sunyer, J O
AU  - Sunyer JO
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Anaphylatoxins)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Anaphylatoxins/*physiology
MH  - Animals
MH  - Cell Movement/immunology/physiology
MH  - Complement System Proteins/*physiology
MH  - Flow Cytometry
MH  - Leukocytes/immunology/physiology
MH  - Oncorhynchus mykiss/immunology/*physiology
MH  - Phagocytes/immunology/*physiology
MH  - Phagocytosis/immunology/*physiology
MH  - Time Factors
EDAT- 2004/07/09 05:00
MHDA- 2005/06/03 09:00
CRDT- 2004/07/09 05:00
PHST- 2003/12/09 00:00 [received]
PHST- 2004/03/17 00:00 [revised]
PHST- 2004/03/22 00:00 [accepted]
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2005/06/03 09:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
AID - S0145305X04000576 [pii]
AID - 10.1016/j.dci.2004.03.004 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2004 Aug;28(10):1005-21. doi: 10.1016/j.dci.2004.03.004.