PMID- 15237407
OWN - NLM
STAT- MEDLINE
DCOM- 20050131
LR  - 20220720
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 25
IP  - 13
DP  - 2004 Jul
TI  - Highly sensitive analysis of multiple pesticides in foods combining solid-phase 
      microextraction, capillary electrophoresis-mass spectrometry, and chemometrics.
PG  - 2065-2076
LID - 10.1002/elps.200405938 [doi]
AB  - A highly sensitive procedure to detect multiple pesticides at trace levels in 
      foods is presented. Initially a comparative study between capillary 
      electrophoresis (CE)-UV and CE-mass spectrometry (MS) is carried out analyzing 
      five pesticides not studied up to now (pyrimethanil, pyrifenox, cyprodinil, 
      cyromazine, and pirimicarb). The comparison between CE-UV and CE-MS is 
      established in terms of separation efficiency, speed of analysis, 
      reproducibility, and sensitivity. A good separation of these compounds is 
      achieved by both techniques using a volatile aqueous buffer containing 0.3 M 
      ammonium acetate/acetic acid at pH 4. Time analysis reproducibility is studied 
      for the same day (n = 5) and three different days (n = 15), showing no 
      significant differences between CE-UV and CE-MS. The study on peak areas 
      reproducibility shows a slightly worse reproducibility for CE-MS compared with 
      CE-UV. The best limit of detection (LOD) that can be achieved for these 
      pesticides using CE-UV was 0.6 microg/mL. CE-MS provides LODs one order of 
      magnitude better than CE-UV. Chemometrics are used to optimize the multiple 
      parameters that play a role in solid-phase microextraction (SPME) and CE-MS 
      analysis (e.g., extraction and desorption times, nebulizer pressure, dry gas 
      flow, dry gas temperature, percentage of organic solvent and acid in the sheath 
      liquid, etc.). The combined use of chemometrics and SPME-CE-MS clearly improves 
      the LODs that can be achieved allowing the detection of pesticides at 
      concentrations down to 15 ng/mL. The usefulness of this approach is demonstrated 
      detecting multiple pesticides in different food samples as grapes and orange 
      juice in a single run. The concentrations detected are below the maximum residue 
      limits (MRLs) permitted for these pesticides in foods corroborating the value of 
      our approach. This work demonstrates, to our knowledge for the first time, the 
      good possibilities of the combined use of SPME-CE-MS and chemometrics.
FAU - Hernandez-Borges, Javier
AU  - Hernandez-Borges J
AD  - Institute of Industrial Fermentations (CSIC), Madrid, Spain.
FAU - Rodriguez-Delgado, Miguel A
AU  - Rodriguez-Delgado MA
AD  - Department of Analytical Chemistry, Nutrition and Food Science, University of La 
      Laguna, La Laguna, Tenerife, Spain.
FAU - Garcia-Montelongo, Francisco J
AU  - Garcia-Montelongo FJ
AD  - Department of Analytical Chemistry, Nutrition and Food Science, University of La 
      Laguna, La Laguna, Tenerife, Spain.
FAU - Cifuentes, Alejandro
AU  - Cifuentes A
AD  - Institute of Industrial Fermentations (CSIC), Madrid, Spain.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Organic Chemicals)
RN  - 0 (Pesticides)
SB  - IM
MH  - Absorption
MH  - Electrophoresis, Capillary/*methods
MH  - Food Contamination/*analysis
MH  - Mass Spectrometry/*methods
MH  - Nebulizers and Vaporizers
MH  - Organic Chemicals
MH  - Pesticides/*analysis
MH  - Pressure
MH  - Reproducibility of Results
MH  - Temperature
MH  - Time Factors
EDAT- 2004/07/09 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/07/09 05:00
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
AID - 10.1002/elps.200405938 [doi]
PST - ppublish
SO  - Electrophoresis. 2004 Jul;25(13):2065-2076. doi: 10.1002/elps.200405938.