PMID- 15238173
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20171116
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 16
IP  - 7
DP  - 2004 Jul
TI  - [Role of bcl-2 and bax expression in apoptosis of hypoxic pulmonary artery smooth 
      muscle cells of rats induced by Na+/H+ exchanger isoform-1 inhibition in vitro].
PG  - 394-8
AB  - OBJECTIVE: To investigate the role of bcl-2 and bax expression in apoptosis of 
      hypoxic pulmonary artery smooth muscle cells (PASMCs) of rats induced by Na+/H+ 
      exchanger isoform-1 (NHE-1) inhibition in vitro. METHODS: Cultured rat PASMCs 
      transfected with NHE-1 ribozyme gene (PRZ cell), cultured rat PASMCs transfected 
      only with pLXSN vector (PX cell) and non-transfected rat PASMCs (PA cell) were 
      obtained in our previous studies. The cells were cultured under low oxygen 
      tension (<1% O2) for 0, 2, 6, 12, 24 and 48 hours respectively. Then cell 
      apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL). Intracellular Ca2+([Ca2+]i) of cells was measured 
      using fluorescence dye Fura-2/AM. The changes in mRNA expression of bcl-2 and bax 
      in cells were assessed by semi quantitative reverse transcription-polymerase 
      chain reaction (sqRT-PCR). The expression of Bcl-2 protein and Bax protein of 
      cells was detected immunohistochemically. RESULTS: In contrast to normal PASMCs 
      and cells transfected with pLXSN, the apoptosis rates in recombinant vectors 
      transfected cells increased significantly and continuously. [Ca2+]i values were 
      also increased in these cells, but the increase extent was remarkably lower than 
      in the normal PASMCs (all P<0.01). The expression of bax mRNA and protein of 
      cells transfected with recombinant vectors was increased significantly compared 
      with cells of other two groups. Meanwhile, the expression of bcl-2 mRNA and 
      protein of these cells were decreased significantly. CONCLUSION: The NHE-1 
      specific hammerhead ribozymes induce apoptosis of PASMCs under low oxygen tension 
      by inhibiting the increase in intracellular Ca2+ concentration, increasing bax 
      expression and decreasing bcl-2 expression.
FAU - Lu, Jun-Yu
AU  - Lu JY
AD  - Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China.
FAU - Yao, Wei
AU  - Yao W
FAU - Qian, Gui-Sheng
AU  - Qian GS
FAU - Yang, Xiao-Jing
AU  - Yang XJ
FAU - Chen, Wei-Zhong
AU  - Chen WZ
FAU - Li, Shu-Ping
AU  - Li SP
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Hypoxia/physiology
MH  - Cells, Cultured
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/metabolism/*pathology
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Pulmonary Artery/cytology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium-Hydrogen Exchangers/*genetics
MH  - Transfection
MH  - bcl-2-Associated X Protein/*metabolism
EDAT- 2004/07/09 05:00
MHDA- 2009/09/09 06:00
CRDT- 2004/07/09 05:00
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004 Jul;16(7):394-8.