PMID- 15241015
OWN - NLM
STAT- MEDLINE
DCOM- 20050214
LR  - 20220716
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 12
IP  - 4
DP  - 2004
TI  - A lineage-specific centromeric satellite sequence in the genus Trifolium.
PG  - 357-67
AB  - We report the molecular structure, genomic organization, chromosomal distribution 
      and evolutionary dynamics of TrR350, a satellite DNA isolated from the forage 
      legume white clover (Trifolium repens L.; 2n = 4 x = 32). The basic repeating 
      unit is an A+T rich 350 bp Hin dIII fragment with a complex dimeric structure 
      consisting of an internal direct repeat of 156 bp packed between unrelated 
      flanking sequences. Each 156 bp repeat has a conserved 24 bp motif repeating at 
      two places. Most of the 24 bp short repeating units enclose a pentanucleotide 
      CAAAA motif, presumed to be involved in breakage-reunion mechanism of tandemly 
      repeating arrays. The dimers share high sequence homology among themselves while 
      monomers within dimers show significant sequence divergence. Genomic Southern 
      hybridization and/or fluorescence in situ hybridization (FISH) on 17 Trifolium 
      species/subspecies revealed that it is a lineage-specific repeat confined to 
      several species within the section Lotoidea originating in the Mediterranean 
      region. The uniform length of the basic repeating unit and the centromeric 
      localization in most of the species harbouring it reflects its extensive 
      conservation in the lineage. However, the Hin dIII restriction profile in seven 
      species also indicated independent evolution of this repeat.
FAU - Ansari, Helal A
AU  - Ansari HA
AD  - AgResearch Grasslands, Private Bag 11008, Palmerston North, New Zealand. 
      helal.ansari@agresearch.co.nz
FAU - Ellison, Nick W
AU  - Ellison NW
FAU - Griffiths, Andrew G
AU  - Griffiths AG
FAU - Williams, Warren M
AU  - Williams WM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Centromere/*genetics
MH  - DNA, Plant/*genetics
MH  - DNA, Satellite/*genetics
MH  - Evolution, Molecular
MH  - In Situ Hybridization, Fluorescence
MH  - Minisatellite Repeats/*genetics
MH  - Species Specificity
MH  - Trifolium/classification/*genetics
EDAT- 2004/07/09 05:00
MHDA- 2005/02/16 09:00
CRDT- 2004/07/09 05:00
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
AID - 5270337 [pii]
AID - 10.1023/B:CHRO.0000034099.19570.b7 [doi]
PST - ppublish
SO  - Chromosome Res. 2004;12(4):357-67. doi: 10.1023/B:CHRO.0000034099.19570.b7.