PMID- 15241349
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20061115
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 114
IP  - 1
DP  - 2004 Jul
TI  - Dendritic cells modulated by cytokine-expressing adenoviruses alleviate 
      eosinophilia and airway hyperresponsiveness in an animal model of asthma.
PG  - 88-96
AB  - BACKGROUND: It has been found that TH1-related cytokines can decrease the 
      accumulation of eosinophils in lung tissue and relieve airway constriction. 
      OBJECTIVE: Dendritic cells (DCs) have been found to prime naive T-helper cells 
      efficiently. In this study, DCs infected with TH1 cytokine-expressing adenovirus 
      can be used to induce antigen-specific TH1 cells for treatment in an animal model 
      of asthma. METHODS: Cytokine gene-modulated DCs pulsed with ovalbumin antigen 
      (OVA) were injected intravenously into naive mice 1 week before sensitization 
      with OVA antigen. The mice were then monitored for OVA-specific IgE, airway 
      inflammatory cell infiltration, and airway hyperresponsiveness in the study. 
      RESULTS: Significant levels of IL-12 or IL-18 were expressed by Ad-IL-12 or 
      Ad-IL-18 infected, bone marrow-derived DCs. Ad-IL-12 and Ad-IL-18 co-infected DCs 
      effectively, decreasing sera anti-OVA IgE antibody levels, lung eosinophilia, and 
      airway hyperresponsiveness. CONCLUSION: We concluded that DCs modulated by 
      TH1-prone cytokine-expressing adenoviruses can alleviate TH2-type airway 
      inflammation in a murine model and can provide possible therapeutic application 
      for DCs in asthma.
CI  - Copyright 2004 American Academy of Allergy, Asthma and Immunology
FAU - Ye, Yi-Ling
AU  - Ye YL
AD  - Department of Medical Technology, Chung Hwa College of Medical Technology, 
      Tainan, Taiwan, Republic of China.
FAU - Lee, Yueh-Lun
AU  - Lee YL
FAU - Chuang, Zen-Jai
AU  - Chuang ZJ
FAU - Lai, Huai-Jean
AU  - Lai HJ
FAU - Chen, Chun-Chi
AU  - Chen CC
FAU - Tao, Mi-Hua
AU  - Tao MH
FAU - Chiang, Bor-Luen
AU  - Chiang BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-18)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adenoviridae/*immunology
MH  - Animals
MH  - Asthma/complications/*immunology
MH  - Bronchial Hyperreactivity/complications/*immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology
MH  - Eosinophilia/complications/*immunology
MH  - Interleukin-12/immunology
MH  - Interleukin-18/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Animal
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
EDAT- 2004/07/09 05:00
MHDA- 2004/09/29 05:00
CRDT- 2004/07/09 05:00
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
AID - S0091674904011418 [pii]
AID - 10.1016/j.jaci.2004.03.010 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2004 Jul;114(1):88-96. doi: 10.1016/j.jaci.2004.03.010.