PMID- 1524216
OWN - NLM
STAT- MEDLINE
DCOM- 19921013
LR  - 20190627
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 203
IP  - 1
DP  - 1992 May 15
TI  - Intramolecularly quenched fluorogenic peptide substrates for human renin.
PG  - 39-46
AB  - Six intramolecularly quenched fluorogenic peptides related to the sequences Phe8 
      to His13, His6 to His13, and Tyr4 to His13 of the human angiotensinogen, 
      containing o-aminobenzoyl (Abz) and ethylenediamine dinitrophenyl (EDDnp) groups 
      at amino- and carboxyl-terminal amino acids residues, were synthesized by 
      classical solution methods. The Leu-Val is the only bond of all obtained peptides 
      that was hydrolyzed by human renin with different degrees of purity and was 
      resistant to hydrolysis by pig renin and cathepsin D. The hydrolysis of 
      Abz-His-Pro-Phe-His-Leu-Val-Ile-His-EDDnp by human renin was inhibited by a 
      highly specific transition-state analog of angiotensinogen (IC50 = 7.8 x 10(-9) 
      M), described by K. Iizuka et al. (1990, J. Med. Chem. 33, 2707-2714). Therefore, 
      specific and sensitive substrates for the continuous assay of human renin in 
      which as little as 70 microGU of human renin could be detected by 
      Abz-Phe-His-Leu-Val-Ile-His-EDDnp were described. The optimal pHs of hydrolysis 
      of the substrates were in the range 4 to 6.
FAU - Oliveira, M C
AU  - Oliveira MC
AD  - Department of Biophysics, Escola Paulista de Medicina, Sao Paulo, Brazil.
FAU - Hirata, I Y
AU  - Hirata IY
FAU - Chagas, J R
AU  - Chagas JR
FAU - Boschcov, P
AU  - Boschcov P
FAU - Gomes, R A
AU  - Gomes RA
FAU - Figueiredo, A F
AU  - Figueiredo AF
FAU - Juliano, L
AU  - Juliano L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Peptides)
RN  - EC 3.4.23.15 (Renin)
RN  - EC 3.4.23.5 (Cathepsin D)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cathepsin D/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Fluorometry
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/*metabolism
MH  - Renin/antagonists & inhibitors/chemistry/*metabolism
MH  - Substrate Specificity
MH  - Swine
EDAT- 1992/05/15 00:00
MHDA- 1992/05/15 00:01
CRDT- 1992/05/15 00:00
PHST- 1992/05/15 00:00 [pubmed]
PHST- 1992/05/15 00:01 [medline]
PHST- 1992/05/15 00:00 [entrez]
AID - 0003-2697(92)90040-E [pii]
AID - 10.1016/0003-2697(92)90040-e [doi]
PST - ppublish
SO  - Anal Biochem. 1992 May 15;203(1):39-46. doi: 10.1016/0003-2697(92)90040-e.