PMID- 15242811
OWN - NLM
STAT- MEDLINE
DCOM- 20040830
LR  - 20220331
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 68
IP  - 3
DP  - 2004 Aug 1
TI  - Dendritic cells maturation promoted by M1 and M4, end products of steroidal 
      ginseng saponins metabolized in digestive tracts, drive a potent Th1 
      polarization.
PG  - 441-52
AB  - Ginseng is a medicinal herb widely used in Asian countries, and many of its 
      pharmacological actions are attributed to the ginsenosides. Dendritic cells (DCs) 
      play a pivotal in the initiation of T-cell-mediated immune responses, making them 
      an attractive cellular adjuvant for use in cancer vaccines. In this study, we 
      investigated whether M1 and M4, end products of steroidal ginseng saponins 
      metabolized in digestive tracts, can drive DCs maturation from human monocytes in 
      vitro. Human monocytes were cultured with GM-CSF and IL-4 for 6 days, followed by 
      another 2 days in the presence of M1, M4 or TNF-alpha as a maturation stimulus. 
      Stimulation with 20 microM of M1 or M4 increased expression level of CD80, CD83 
      and CD86 as expressed by mean fluorescence intensity (MFI) and decreased 
      endocytic activity. M4-primed mature DCs also displayed enhanced T cells 
      stimulatory capacity in a MLR, as measured by T cell proliferation. Mature DCs 
      differentiated with M1 or M4 induced the differentiation of naive T cells towards 
      a helper T cell type 1 (Th1) response at DC/T (1:5) cells ratio depending on 
      IL-12 secretion. In CTL assay, the production of IFN-gamma and 51Cr release on 
      M4-primed mature DCs was more augmented than of immature DCs or TNF-alpha-primed 
      mature DCs. These results suggest that M4 may be used on DC-based vaccines for 
      cancer immunotherapy.
FAU - Takei, Masao
AU  - Takei M
AD  - Division of Cellular Allergology, Research Center Borstel, Parkallee 22 D-23845, 
      Germany. mtakei@fz-borstel.de
FAU - Tachikawa, Eiichi
AU  - Tachikawa E
FAU - Hasegawa, Hideo
AU  - Hasegawa H
FAU - Lee, Je-Jung
AU  - Lee JJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Interleukin-6)
RN  - 0 (Protein Subunits)
RN  - 0 (Saponins)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/cytology/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cellular Senescence/*drug effects
MH  - Dendritic Cells/*drug effects/physiology
MH  - Humans
MH  - Interleukin-12/metabolism
MH  - Interleukin-6/metabolism
MH  - Leukocytes/drug effects/metabolism
MH  - Panax/*chemistry
MH  - Phenotype
MH  - Protein Subunits/metabolism
MH  - Saponins/metabolism/*pharmacology
MH  - T-Lymphocytes/cytology/drug effects
MH  - Th1 Cells/cytology/*drug effects
EDAT- 2004/07/10 05:00
MHDA- 2004/08/31 05:00
CRDT- 2004/07/10 05:00
PHST- 2003/12/08 00:00 [received]
PHST- 2004/04/22 00:00 [accepted]
PHST- 2004/07/10 05:00 [pubmed]
PHST- 2004/08/31 05:00 [medline]
PHST- 2004/07/10 05:00 [entrez]
AID - S0006295204002618 [pii]
AID - 10.1016/j.bcp.2004.04.015 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 2004 Aug 1;68(3):441-52. doi: 10.1016/j.bcp.2004.04.015.