PMID- 15242941
OWN - NLM
STAT- MEDLINE
DCOM- 20050113
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 11
IP  - 4
DP  - 2004 Jul
TI  - Allergenic characterization of tropomyosin from the dusky brown cockroach, 
      Periplaneta fuliginosa.
PG  - 680-5
AB  - Household arthropods are one of the most common causes of allergic diseases. Four 
      species of cockroaches are found to reside in Korean homes, but published work 
      deals almost exclusively with the German and American cockroaches. This study was 
      undertaken to investigate the cross-reactive allergenic components of the dusky 
      brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay 
      (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were 
      performed with Blattella germanica and Dermatophagoides farinae allergic sera. 
      cDNA encoding tropomyosin, which is a well known cross-reactive pan-allergen, was 
      cloned by reverse transcriptase PCR, and recombinant protein was produced by 
      using a pET-28b expression system. Native tropomyosin was purified by ammonium 
      sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities 
      of native and recombinant tropomyosins were compared by an ELISA inhibition 
      study. All 30 sera tested showed P. fuliginosa-specific IgE, and the IgE-binding 
      reactivity of the P. fuliginosa extract was inhibited as much as 79.4% by a B. 
      germanica extract and as much as 63.3% by a D. farinae extract. The deduced amino 
      acid sequence of cloned cDNA was identical with that of Periplaneta americana 
      tropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic 
      sera had IgE specific for recombinant protein, and the maximum inhibition of P. 
      fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an 
      inhibitor concentration of 10 microg/ml. Native tropomyosin inhibited the binding 
      of IgE to the P. fuliginosa, B. germanica, and D. farinae extracts by 65.0, 51.8, 
      and 39% at an inhibitor concentration of 1 microg/ml. P. fuliginosa appears to 
      possess allergens that are highly cross-reactive with allergens of B. germanica 
      and D. farinae. Tropomyosin was found to be a major allergenic component 
      accounting for the cross-reactivity between cockroaches and dust mites.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AD  - Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 
      Project for Medical Science, Yonsei University College of Medicine, Seoul 
      120-752, Korea.
FAU - Hwang, Heeyu
AU  - Hwang H
FAU - Lee, Jongweon
AU  - Lee J
FAU - Lee, In-Yong
AU  - Lee IY
FAU - Kim, Dong Soo
AU  - Kim DS
FAU - Hong, Chein-Soo
AU  - Hong CS
FAU - Ree, Han-Il
AU  - Ree HI
FAU - Yong, Tai-Soon
AU  - Yong TS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
EIN - Clin Diagn Lab Immunol. 2006 May;13(5):602
MH  - Adolescent
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Child
MH  - Cloning, Molecular
MH  - Cross Reactions
MH  - DNA, Complementary/analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/*immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Periplaneta/genetics/*immunology
MH  - Recombinant Proteins/genetics/*immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology
MH  - Species Specificity
MH  - Tropomyosin/*genetics/*immunology
PMC - PMC440615
EDAT- 2004/07/10 05:00
MHDA- 2005/01/14 09:00
PMCR- 2004/07/01
CRDT- 2004/07/10 05:00
PHST- 2004/07/10 05:00 [pubmed]
PHST- 2005/01/14 09:00 [medline]
PHST- 2004/07/10 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - 11/4/680 [pii]
AID - 0073-04 [pii]
AID - 10.1128/CDLI.11.4.680-685.2004 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2004 Jul;11(4):680-5. doi: 
      10.1128/CDLI.11.4.680-685.2004.