PMID- 15244481
OWN - NLM
STAT- MEDLINE
DCOM- 20050209
LR  - 20171116
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 5
IP  - 4
DP  - 2004 Jul-Aug
TI  - Protamine assembled in multilayers on colloidal particles can be exchanged and 
      released.
PG  - 1580-7
AB  - Biocomposite thin films assembled on colloidal particles by means of 
      layer-by-layer adsorption have been suggested as drug carriers and diagnostic 
      devices. Protamine (PRM)/dextransulfate (DXS) and protamine/bovine serum albumine 
      (BSA) multilayers were fabricated on colloidal silica and subsequently 
      investigated by means of fluorescence activated cell sorting (FACS) and 
      microelectrophoresis. Fluorescein labeled polyelectrolytes were embedded at 
      different positions in the multilayers as a marker for layer growth. FACS showed 
      that PRM and DXS formed regular growing stable multilayers, yet adsorbed PRM can 
      be nevertheless exchanged with PRM in solution during layer formation and also 
      after the multilayer formation has been completed. Up to 90% of the PRM pool was 
      available for exchange. PRM together with BSA as demonstrated by SFM did not form 
      multilayers under the applied conditions although the zeta-potential, commonly 
      used as an indicator for stepwise adsorption, observed characteristic 
      alternations. The capability of bound PRM to exchange with PRM in solution is 
      attributed to its relatively small size. The demonstrated exchange may have 
      importance in designing multilayers with smart release features. Furthermore, 
      FACS proved to be a rather suitable means to quantify the aggregation behavior 
      during coating and washing. Singulets, doublets, triplets, and aggregates of 
      higher order could be clearly resolved. The aggregation of particles coated with 
      PRM/DXS layers was higher than that of silica particles coated with PAH/PSS 
      layers. In the first case about 50% of all recorded events are attributed to 
      aggregats, while the PAH/PSS coating produced only about 10% aggregates.
FAU - Hiller, Sabine
AU  - Hiller S
AD  - Institute of Medical Physics and Biophysics, University of Leipzig, D-04103 
      Leipzig, Germany. reichls@medizin.uni-leipzig.de
FAU - Leporatti, Stefano
AU  - Leporatti S
FAU - Schnackel, Andreas
AU  - Schnackel A
FAU - Typlt, Elke
AU  - Typlt E
FAU - Donath, Edwin
AU  - Donath E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Biocompatible Materials)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Colloids)
RN  - 0 (Dextrans)
RN  - 0 (Protamines)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Cattle
MH  - Coated Materials, Biocompatible/chemistry
MH  - Colloids/*chemistry
MH  - Dextrans/chemistry
MH  - Electrophoresis
MH  - Flow Cytometry/methods
MH  - Protamines/*chemistry
MH  - Serum Albumin, Bovine/chemistry
MH  - Silicon Dioxide/chemistry
EDAT- 2004/07/13 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/07/13 05:00
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
AID - 10.1021/bm049875m [doi]
PST - ppublish
SO  - Biomacromolecules. 2004 Jul-Aug;5(4):1580-7. doi: 10.1021/bm049875m.