PMID- 15245591
OWN - NLM
STAT- MEDLINE
DCOM- 20040831
LR  - 20190513
IS  - 1347-9032 (Print)
IS  - 1347-9032 (Linking)
VI  - 95
IP  - 7
DP  - 2004 Jul
TI  - Prompt tumor formation and maintenance of constitutive NF-kappaB activity of 
      multiple myeloma cells in NOD/SCID/gammacnull mice.
PG  - 564-8
AB  - Clinically and biologically relevant animal models are indispensable to evaluate 
      both the pathophysiology and strategies for diagnosis and treatment of multiple 
      myeloma (MM). We examined the tumorigenicity of MM cell lines KMM-1 and U-266 in 
      an in vivo cell proliferation model using NOD/SCID/gammacnull (NOG) mice. Two 
      cell lines were inoculated either subcutaneously (s.c.) in the post-auricular 
      region or intravenously (i.v.) in the tail of NOG mice. The KMM-1 cell line 
      produced a progressively growing large tumor with infiltration of the cells 
      expressing human lambda-chain in various organs of all NOG mice, while the U-266 
      cell line failed to do so. Tumor cells grown in NOG mice maintained the original 
      histomorphology, as well as expression patterns of tumor markers human lambda Ig 
      light chain and VEGF. Tumor progression in mice also correlated with elevation of 
      serum human soluble IL-6R and gp130. Tumor cells sustained a strong NF-kappaB 
      activity in vivo and induced NF-kappaB components were indistinguishable from 
      those in cells cultured in vitro. The rapid and efficient engraftment of the MM 
      cell line in NOG mice suggests that this is a very useful animal model which 
      could provide a novel system in which to clarify the mechanism of growth of 
      cancer cells, as well as to develop new therapeutic regimens against MM.
FAU - Dewan, Md Zahidunnabi
AU  - Dewan MZ
AD  - Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical 
      and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
FAU - Watanabe, Mariko
AU  - Watanabe M
FAU - Terashima, Kazuo
AU  - Terashima K
FAU - Aoki, Mizuho
AU  - Aoki M
FAU - Sata, Tetsutaro
AU  - Sata T
FAU - Honda, Mitsuo
AU  - Honda M
FAU - Ito, Mamoru
AU  - Ito M
FAU - Yamaoka, Shoji
AU  - Yamaoka S
FAU - Watanabe, Toshiki
AU  - Watanabe T
FAU - Horie, Ryouichi
AU  - Horie R
FAU - Yamamoto, Naoki
AU  - Yamamoto N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - *Disease Models, Animal
MH  - Disease Progression
MH  - Humans
MH  - Mice
MH  - Mice, Inbred NOD
MH  - *Mice, SCID
MH  - Multiple Myeloma/*physiopathology/*veterinary
MH  - NF-kappa B/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2004/07/13 05:00
MHDA- 2004/09/01 05:00
CRDT- 2004/07/13 05:00
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/09/01 05:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
AID - 10.1111/j.1349-7006.2004.tb02487.x [doi]
PST - ppublish
SO  - Cancer Sci. 2004 Jul;95(7):564-8. doi: 10.1111/j.1349-7006.2004.tb02487.x.