PMID- 15245916
OWN - NLM
STAT- MEDLINE
DCOM- 20040916
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1679
IP  - 1
DP  - 2004 Jul 13
TI  - Cloning of zebrafish metallothionein gene and characterization of its gene 
      promoter region in HepG2 cell line.
PG  - 47-58
AB  - The coding region of cDNA and genomic DNA, with its promoter region, of zebrafish 
      metallothionein (zMT) gene homologous to the piscine MT-II was obtained. The 
      A/T-rich promoter region contains four metal regulatory elements (MREs), three 
      activator protein 1 (AP1) and one specific protein 1 (Sp1) binding sites. The 
      four MREs are organized into two clusters, a distal cluster with one MRE lying 
      around 740 bp upstream of the transcription start point and a proximal cluster 
      with three MREs located close to the TATA box. The metal induction ability of the 
      promoter was assessed by transient luciferase gene expression assays in HepG2 
      cells. The zMT promoter was inducible by Zn2+, Cd2+, Cu2+ and Hg2+ ions in 
      decreasing inducibility, while inert to Ni2+, Pb2+ and Co2+ ions, and H2O2 
      treatment in vitro. Deletion of the putative cis-acting elements in the promoter 
      region revealed that the distal MRE (MREd) was important in mediating metal 
      inducibility. Despite the binding of HepG2 cell nuclear protein factors to all 
      MREs as confirmed by electrophoretic mobility shift assay (EMSA), the proximal 
      MREs did not provide significant contribution to metal induction of zMT gene in 
      HepG2 cells. The metal inducibility of zMT promoter required the cooperative 
      effect of at least three MRE sites.
CI  - Copyright 2004 Elsevier B.V.
FAU - Yan, Carol Hiu Mei
AU  - Yan CH
AD  - Department of Biochemistry, The Chinese University of Hong Kong, Sha Tin, N.T., 
      Hong Kong SAR, China.
FAU - Chan, King Ming
AU  - Chan KM
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA Primers)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Electrophoretic Mobility Shift Assay
MH  - Humans
MH  - Metallothionein/*genetics
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Zebrafish
EDAT- 2004/07/13 05:00
MHDA- 2004/09/17 05:00
CRDT- 2004/07/13 05:00
PHST- 2003/12/23 00:00 [received]
PHST- 2004/03/24 00:00 [revised]
PHST- 2004/04/14 00:00 [accepted]
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/09/17 05:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
AID - S0167478104000831 [pii]
AID - 10.1016/j.bbaexp.2004.04.004 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2004 Jul 13;1679(1):47-58. doi: 
      10.1016/j.bbaexp.2004.04.004.