PMID- 15246270
OWN - NLM
STAT- MEDLINE
DCOM- 20040910
LR  - 20200410
IS  - 0042-6822 (Print)
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 325
IP  - 2
DP  - 2004 Aug 1
TI  - A highly pathogenic porcine reproductive and respiratory syndrome virus generated 
      from an infectious cDNA clone retains the in vivo virulence and transmissibility 
      properties of the parental virus.
PG  - 308-19
AB  - The nucleotide sequence of a highly pathogenic porcine reproductive and 
      respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells 
      with capped in vitro transcripts derived from a full-length cDNA clone of the 
      viral genome resulted in infectious PRRSV with growth characteristics similar to 
      that of the parental virus. Primer extension analysis revealed that during 
      replication, the viral polymerase corrected the two nonviral guanosine residues 
      present at the 5' terminus of the transfected transcripts. Animal studies showed 
      that the cloned virus induced hyperthermia, persistent viremia, and antibody 
      response, similar to that observed with the parental virus. Contact transmission 
      occurred rapidly within 3 days of introduction of naive pigs into the group of 
      clone virus-inoculated pigs. These results suggest that the cloned virus retains 
      the in vivo virulence and contagion properties of the parental virus, thus, 
      providing the background for reverse genetics manipulation in systematic 
      examination of attenuation and virulence phenotypes.
FAU - Truong, Ha M
AU  - Truong HM
AD  - Department of Veterinary and Biomedical Sciences and the Nebraska Center for 
      Virology, University of Nebraska, Lincoln, NE 68588, USA.
FAU - Lu, Z
AU  - Lu Z
FAU - Kutish, Gerald F
AU  - Kutish GF
FAU - Galeota, Judith
AU  - Galeota J
FAU - Osorio, Fernando A
AU  - Osorio FA
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
LA  - eng
GR  - AI-34956/AI/NIAID NIH HHS/United States
GR  - R01 AI034956/AI/NIAID NIH HHS/United States
GR  - R29 AI034956/AI/NIAID NIH HHS/United States
GR  - P20RR15635/RR/NCRR NIH HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/*genetics
MH  - Genome, Viral
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/*pathogenicity/physiology
MH  - Sus scrofa
MH  - Virulence/genetics
MH  - Virus Replication
PMC - PMC7127741
EDAT- 2004/07/13 05:00
MHDA- 2004/09/11 05:00
PMCR- 2004/06/17
CRDT- 2004/07/13 05:00
PHST- 2004/02/23 00:00 [received]
PHST- 2004/04/12 00:00 [revised]
PHST- 2004/04/30 00:00 [accepted]
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/09/11 05:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
PHST- 2004/06/17 00:00 [pmc-release]
AID - S0042682204003514 [pii]
AID - S0042-6822(04)00351-4 [pii]
AID - 10.1016/j.virol.2004.04.046 [doi]
PST - ppublish
SO  - Virology. 2004 Aug 1;325(2):308-19. doi: 10.1016/j.virol.2004.04.046.