PMID- 15246649
OWN - NLM
STAT- MEDLINE
DCOM- 20041101
LR  - 20071114
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 104
IP  - 2
DP  - 2004 Sep 1
TI  - Susceptibility of peritoneal macrophages to infection by Theiler's virus.
PG  - 123-7
AB  - Theiler's murine encephalomyelitis virus (TMEV) strains fall into two groups: 
      high-neurovirulence GDVII virus results in rapidly fatal encephalitis, while 
      low-neurovirulence BeAn and DA viruses produce persistent central nervous system 
      (CNS) infection and inflammatory demyelinating disease. Because macrophages 
      (Mphis) are key components in BeAn virus-induced demyelinating disease, we 
      examined the susceptibility of primary peritoneal macrophages (pMphis) to BeAn 
      infection in vitro. Freshly isolated, thioglycollate-elicited pMphis were 
      resistant to BeAn virus infection even at high multiplicity of infection. In 
      contrast, after incubation of thioglycollate-elicited pMphis at 37 degrees C for 
      4 days before infection, approximately half of the cells expressed virus 
      antigen(s) and contained nicked DNA indicative of apoptosis. However, BeAn virus 
      RNA replication and virus yields were highly restricted. Interestingly, about 
      one-third of the cells were apoptotic but negative for virus RNA and antigen(s). 
      Tumor necrosis factor-alpha (TNF-alpha) and interferon-alpha (IFN-alpha) were 
      elevated in BeAn-infected pMphi cultures suggesting that bystander killing may be 
      responsible for the apoptosis seen in BeAn virus antigen-negative cells. These 
      data show for the first time that pMphis are susceptible to BeAn virus infection, 
      although the infection is highly restricted and most of these cells undergo 
      BeAn-induced apoptosis.
FAU - Jelachich, Mary Lou
AU  - Jelachich ML
AD  - Department of Neurology & Microbiology-Immunology, Northwestern University, 2650 
      N. Ridge Ave., Evanston, IL 60201, USA.
FAU - Reddi, Honey V
AU  - Reddi HV
FAU - Trottier, Mark D
AU  - Trottier MD
FAU - Schlitt, Brian P
AU  - Schlitt BP
FAU - Lipton, Howard L
AU  - Lipton HL
LA  - eng
GR  - P01 NS23349/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Line
MH  - Macrophages, Peritoneal/cytology/physiology/*virology
MH  - Mice
MH  - Theilovirus/*pathogenicity/physiology
EDAT- 2004/07/13 05:00
MHDA- 2004/11/02 09:00
CRDT- 2004/07/13 05:00
PHST- 2003/11/20 00:00 [received]
PHST- 2004/03/10 00:00 [revised]
PHST- 2004/03/10 00:00 [accepted]
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/11/02 09:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
AID - S0168170204001789 [pii]
AID - 10.1016/j.virusres.2004.03.006 [doi]
PST - ppublish
SO  - Virus Res. 2004 Sep 1;104(2):123-7. doi: 10.1016/j.virusres.2004.03.006.