PMID- 15246654
OWN - NLM
STAT- MEDLINE
DCOM- 20041101
LR  - 20200423
IS  - 0168-1702 (Print)
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 104
IP  - 2
DP  - 2004 Sep 1
TI  - Molecular characterization of PL97-1, the first Korean isolate of the porcine 
      reproductive and respiratory syndrome virus.
PG  - 165-79
AB  - We determined the complete nucleotide and predicted amino acid sequence of the 
      genomic RNA of PL97-1, the first Korean strain of porcine reproductive and 
      respiratory syndrome virus (PRRSV), which was isolated from the serum of an 
      infected pig in 1997. We found that the 15411-nucleotide genome of PL97-1 
      consisted of a 189-nucleotide 5' noncoding region (NCR), a 15071-nucleotide 
      protein-coding region, and a 151-nucleotide 3'NCR, followed by a poly (A) tail. 
      The 5'-end of PL97-1 began with 1ATG ACG TAT AGG12. Comparison of the PL97-1 
      genome with the 11 fully sequenced PRRSV genomes currently available revealed 
      sequence divergence ranging from 0.3% (the VR-2332-derived vaccine MLV 
      RespPRRS/Repro strain) to 38% (the Dutch Lelystad strain). To better understand 
      the genetic relationships between these different strains, phylogenetic analyses 
      were performed on the full-length PRRSV genomes. Significantly, the phylogenetic 
      tree based on the ORF1b or ORF7 genes most closely resembled the tree based on 
      the full-length genomes. Thus, these single genes will be the most useful in 
      revealing the genetic relationships between the different strains relative to 
      their geographical distribution. Extensive phylogenetic analyses using the ORF7 
      sequences of 111 PRRSV isolates available revealed that PL97-1 is most closely 
      related to the North American genotype VR-2332, a VR-2332-derived vaccine strain, 
      and Chinese BJ-4. It is distantly related to the European genotype Lelystad. This 
      study provides the largest full-length genome phylogenetic analysis of PRRSV that 
      has been published to date, and supports an earlier genetic grouping of the many 
      temporally and geographically diverse PRRSV strains currently isolated.
FAU - Kang, Shien-Young
AU  - Kang SY
AD  - Research Institute of Veterinary Medicine, College of Veterinary Medicine, 
      Chungbuk National University, Cheongju, South Korea.
FAU - Yun, Sang-Im
AU  - Yun SI
FAU - Park, Hyo-Sun
AU  - Park HS
FAU - Park, Choi-Kyu
AU  - Park CK
FAU - Choi, Hyo-Seong
AU  - Choi HS
FAU - Lee, Young-Min
AU  - Lee YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Genome, Viral
MH  - Korea/epidemiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*classification/genetics
MH  - RNA, Viral/analysis/genetics
PMC - PMC7172542
EDAT- 2004/07/13 05:00
MHDA- 2004/11/02 09:00
PMCR- 2004/06/19
CRDT- 2004/07/13 05:00
PHST- 2003/12/30 00:00 [received]
PHST- 2004/04/05 00:00 [revised]
PHST- 2004/04/05 00:00 [accepted]
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/11/02 09:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
PHST- 2004/06/19 00:00 [pmc-release]
AID - S0168170204001868 [pii]
AID - 10.1016/j.virusres.2004.04.003 [doi]
PST - ppublish
SO  - Virus Res. 2004 Sep 1;104(2):165-79. doi: 10.1016/j.virusres.2004.04.003.