PMID- 15246982
OWN - NLM
STAT- MEDLINE
DCOM- 20040817
LR  - 20220317
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 287
IP  - 2
DP  - 2004 Aug
TI  - Accessory cell function of airway epithelial cells.
PG  - L318-31
AB  - Accessory cell function of airway epithelial cells. We previously demonstrated 
      that airway epithelial cells (AECs) have many features of accessory cells, 
      including expression of class II molecules CD80 and CD86 and functional Fcgamma 
      receptors. We have extended these studies to show that freshly isolated AECs have 
      mRNA for cathepsins S, V, and H [proteases important in antigen (Ag) 
      presentation], invariant chain, human leukocyte antigen (HLA)-DM-alpha and 
      HLA-DM-beta, and CLIP, an invariant chain breakdown product. A physiologically 
      relevant Ag, ragweed, was colocalized with HLA-DR in AECs, and its uptake was 
      increased by granulocyte-macrophage colony-stimulating factor and IFN-gamma 
      treatments, which had no effect on CD80 and CD86 expression. We demonstrate the 
      presence of other costimulatory molecules, including B7h and B7-H1, on AECs and 
      the increased expression of B7-H1 on AECs after treatment with 
      granulocyte-macrophage colony-stimulating factor and IFN-gamma. Finally, we 
      compared T cell proliferation after allostimulation with AECs and dendritic cells 
      (DCs). The precursor frequency of peripheral blood T cells responding to AECs was 
      0.264% compared with 0.55% for DCs. DCs stimulated CD45RO(+), CD45RA(+), CCR7(+) 
      and CCR7(-)CD4(+), and CD8(+) T cells, whereas AECs stimulated only CD45RO(+), 
      CD45RA(-), CCR7(-), CD4(+), and CD8(+) T cells. There was no difference in 
      cytokine production, type of memory T cells stimulated (effector vs. long-term 
      memory), or apoptosis by T cells cocultured with AECs and DCs. The localization 
      of AECs exposed to the external environment may make them important in the 
      regulation of local immune responses.
FAU - Oei, Erwin
AU  - Oei E
AD  - Division of Pulmonary and Critical Care Medicine, Mount Sinai School of Medicine, 
      New York, NY, USA.
FAU - Kalb, Thomas
AU  - Kalb T
FAU - Beuria, Prarthana
AU  - Beuria P
FAU - Allez, Matthieu
AU  - Allez M
FAU - Nakazawa, Atsushi
AU  - Nakazawa A
FAU - Azuma, Miyuki
AU  - Azuma M
FAU - Timony, Michael
AU  - Timony M
FAU - Stuart, Zanetta
AU  - Stuart Z
FAU - Chen, Houchu
AU  - Chen H
FAU - Sperber, Kirk
AU  - Sperber K
LA  - eng
GR  - AI-44236/AI/NIAID NIH HHS/United States
GR  - AI-45343/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Allergens)
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CCR7 protein, human)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, Chemokine)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.4.- (Cathepsins)
SB  - IM
MH  - Allergens/immunology
MH  - Ambrosia/immunology
MH  - Antigens, CD/metabolism
MH  - Apoptosis/immunology
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cathepsins/genetics
MH  - Cell Line
MH  - Cytokines/pharmacology
MH  - Dendritic Cells/*immunology
MH  - HLA-D Antigens/metabolism
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Immunologic Memory/immunology
MH  - Leukocyte Common Antigens/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Nasal Mucosa/*cytology/*immunology/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, CCR7
MH  - Receptors, Chemokine/genetics
EDAT- 2004/07/13 05:00
MHDA- 2004/08/18 05:00
CRDT- 2004/07/13 05:00
PHST- 2004/07/13 05:00 [pubmed]
PHST- 2004/08/18 05:00 [medline]
PHST- 2004/07/13 05:00 [entrez]
AID - 287/2/L318 [pii]
AID - 10.1152/ajplung.00174.2003 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2004 Aug;287(2):L318-31. doi: 
      10.1152/ajplung.00174.2003.