PMID- 15248914
OWN - NLM
STAT- MEDLINE
DCOM- 20050125
LR  - 20150313
VI  - 23
IP  - 7
DP  - 2004 Jul
TI  - [Diagnosis of lymph node micrometastases in human lung carcinoma: determination 
      of p53 gene mutations by polymerase chain reaction-temperature gradient gel 
      electrophoresis (PCR-TGGE)].
PG  - 794-8
AB  - BACKGROUND & OBJECTIVE: Metastasis is one of the most important factors in 
      determining the prognosis of lung cancer patients. However, it is difficult to 
      determine single cancer cell in lymph nodes by routine methods. This study was 
      designed to investigate a sensitive method for detecting lymph node 
      micrometastases in human lung carcinomas. METHODS: Mutation detection for p53 
      gene (exon 5-8) was performed in 39 cases of primary lung carcinomas and 110 
      corresponding lymph nodes (LNS) by PCR-TGGE method. Serial sectioning was 
      performed to confirm micrometastases in some of these samples. RESULTS: p53 
      mutations were found in 23 of 39 cases with primary lung cancer. Forty LNSs 
      showed p53 mutation in 67 corresponding LNSs. They were located at the same exon 
      as their primary tumors. Only twenty-six LNSs were detected metastases by routine 
      histopathology. Forty-three LNSs from sixteen cases of primary tumor without p53 
      mutation and ten LNSs from the patients without tumor disease did not have any 
      p53 mutations. By PCR-TGGE assay p53 gene mutations were determined in fourteen 
      LNSs without histopathological evidence of metastasis. Micrometastases were found 
      in four of the serially sectioned cases. CONCLUSION: Mutation detection for p53 
      gene (exon 5-8) showed lymph node micrometastases in patients with lung 
      carcinoma. It may be used as a complement for the routine histopathology.
FAU - Xu, Mei-Lin
AU  - Xu ML
AD  - Department of Pathology, Tianjin Chest Hospital, Tianjin, 300051, P.R.China. 
      meilinxu-gh@163.com
FAU - Wu, Bing-Quan
AU  - Wu BQ
FAU - Zhong, Hao-Hao
AU  - Zhong HH
FAU - Yang, Xia
AU  - Yang X
FAU - Heng, Wan-Jie
AU  - Heng WJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
SB  - IM
MH  - Adenocarcinoma/genetics/secondary
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/genetics/secondary
MH  - Electrophoresis, Agar Gel
MH  - Female
MH  - *Genes, p53
MH  - Humans
MH  - Lung Neoplasms/genetics/*pathology
MH  - Lymph Nodes/*pathology
MH  - Lymphatic Metastasis/*diagnosis
MH  - Male
MH  - Middle Aged
MH  - *Mutation
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
EDAT- 2004/07/14 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/07/14 05:00
PHST- 2004/07/14 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/07/14 05:00 [entrez]
AID - 1000467X2004070794 [pii]
PST - ppublish
SO  - Ai Zheng. 2004 Jul;23(7):794-8.