PMID- 15249685
OWN - NLM
STAT- MEDLINE
DCOM- 20040826
LR  - 20240411
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 101
IP  - 29
DP  - 2004 Jul 20
TI  - Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of 
      TRIM5alpha.
PG  - 10774-9
AB  - Mammalian cells express several factors that act in a cell-autonomous manner to 
      inhibit retrovirus replication. Among these are the Friend virus susceptibility 
      factor 1/lentivirus susceptibility factor 1/restriction factor 1 (Ref1) class of 
      restriction factors, which block infection by targeting the capsids of diverse 
      retroviruses. Here we show that lentivirus susceptibility factor 1 and Ref1 are 
      species-specific variants of tripartite interaction motif 5alpha (TRIM5alpha), a 
      cytoplasmic body component recently shown to block HIV-1 infection in rhesus 
      macaque cells, and can indeed block infection by widely divergent retroviruses. 
      Depletion of TRIM5alpha from human cells relieved restriction of N-tropic murine 
      leukemia virus (N-MLV), and expression of human TRIM5alpha in otherwise 
      nonrestricting cells conferred specific resistance to N-MLV infection, indicating 
      that TRIM5alpha is Ref1 or an essential component of Ref1. TRIM5alpha variants 
      from humans, rhesus monkeys, and African green monkeys displayed different but 
      overlapping restriction specificities that were quite accurately predicted by the 
      restriction properties of the cells from which they were derived. All TRIM5alpha 
      variants could inhibit infection by at least two different retroviruses, and 
      African green monkey TRIM5alpha was able to inhibit infection by no less than 
      four divergent retroviruses of human, non-human primate, equine, and murine 
      origin. However, each TRIM5alpha variant was unable to restrict retroviruses 
      isolated from the same species. These data indicate that TRIM5alpha can confer 
      broad innate immunity to retrovirus infection in primate cells and is likely to 
      be an important natural barrier to cross-species retrovirus transmission.
FAU - Hatziioannou, Theodora
AU  - Hatziioannou T
AD  - Aaron Diamond AIDS Research Center, New York, NY 10016, USA.
FAU - Perez-Caballero, David
AU  - Perez-Caballero D
FAU - Yang, Annie
AU  - Yang A
FAU - Cowan, Simone
AU  - Cowan S
FAU - Bieniasz, Paul D
AU  - Bieniasz PD
LA  - eng
SI  - GENBANK/AY669399
GR  - R01 AI050111/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040712
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antiviral Agents)
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
CIN - Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10496-7. PMID: 15252204
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antiviral Agents/genetics/*metabolism
MH  - Antiviral Restriction Factors
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Humans
MH  - Macaca mulatta
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics/metabolism
MH  - Retroviridae/*metabolism
MH  - Retroviridae Infections/metabolism
MH  - Sequence Alignment
MH  - Species Specificity
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases
PMC - PMC490010
EDAT- 2004/07/14 05:00
MHDA- 2004/08/27 05:00
PMCR- 2005/01/20
CRDT- 2004/07/14 05:00
PHST- 2004/07/14 05:00 [pubmed]
PHST- 2004/08/27 05:00 [medline]
PHST- 2004/07/14 05:00 [entrez]
PHST- 2005/01/20 00:00 [pmc-release]
AID - 0402361101 [pii]
AID - 10110774 [pii]
AID - 10.1073/pnas.0402361101 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10774-9. doi: 
      10.1073/pnas.0402361101. Epub 2004 Jul 12.