PMID- 1525180
OWN - NLM
STAT- MEDLINE
DCOM- 19921020
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1117
IP  - 2
DP  - 1992 Sep 15
TI  - Purification of a growth factor from pigeon milk.
PG  - 193-8
AB  - Aqueous extracts of pigeon milk (PM) stimulated the in vitro growth of quiescent 
      CHO cells maintained in culture and caused precocial incisor eruption and eyelid 
      opening in newborn mice. CM-cellulose chromatography of the growth factor of PM 
      enhanced its biologic activity. Rechromatography of CM-active fractions in 
      DEAE-cellulose columns yielded a single peak that was mitogenic both in vivo and 
      in vitro. SDS-PAGE of DEAE-active fractions gave rise to a single band of 
      molecular weight about 6000. This protein, termed as pigeon milk-derived growth 
      factor (PMGF), yielded a single peak on gel filtration in Sephadex G-100 columns. 
      Elution pattern of PMGF was similar to that obtained for epidermal growth factor 
      of mouse submaxillary gland (mEGF). The chromatographic plus electrophoretic 
      pattern, and biologic action of PMGF suggest it to be a EGF-like protein.
FAU - Shetty, S
AU  - Shetty S
AD  - Department of Biosciences, Mangalore University, Mangalagangotri, India.
FAU - Hegde, S N
AU  - Hegde SN
FAU - Bharathi, L
AU  - Bharathi L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Growth Substances)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/growth & development
MH  - CHO Cells/cytology
MH  - Chromatography
MH  - Chromatography, Gel
MH  - Columbidae/*metabolism
MH  - Cricetinae
MH  - Crop, Avian/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Growth Substances/*isolation & purification/pharmacology
MH  - Mice
MH  - Milk/analysis
EDAT- 1992/09/15 00:00
MHDA- 1992/09/15 00:01
CRDT- 1992/09/15 00:00
PHST- 1992/09/15 00:00 [pubmed]
PHST- 1992/09/15 00:01 [medline]
PHST- 1992/09/15 00:00 [entrez]
AID - 0304-4165(92)90079-A [pii]
AID - 10.1016/0304-4165(92)90079-a [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1992 Sep 15;1117(2):193-8. doi: 
      10.1016/0304-4165(92)90079-a.