PMID- 15252239
OWN - NLM
STAT- MEDLINE
DCOM- 20050209
LR  - 20181113
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 12
IP  - 5
DP  - 2004
TI  - Behaviour of nucleolus organizing regions (NORs) and nucleoli during mitotic and 
      meiotic divisions in budding yeast.
PG  - 427-38
AB  - Spatial organization and segregation behaviour in mitosis and meiosis of nucleoli 
      and NORs of Saccharomyces cerevisiae were examined by fluorescence in situ 
      hybridization (FISH) and immunostaining, and integrated with previous 
      ultrastructural studies. Our observations suggest that in interphase the 
      NOR-bearing chromosome arm forms a hairpin-shaped loop inside the nucleolus, 
      unlike the other arms which adopt a Rabl-like orientation. Prior to mitosis and 
      meiosis, the appearance of the NOR changes from puffed to thread-shaped. In 
      mitosis, it is stretched between the mother and daughter nuclei and seems to be 
      among the last regions where chromatids separate. The nucleoli remain intact and 
      split at the end of anaphase. Similarly, during meiosis I, intact nucleoli trail 
      behind the separating homologous NORs and are partitioned equally to the two 
      half-nuclei. During the second meiotic division, however, the nucleolus, together 
      with a major portion of the nucleoplasm and the nuclear pore complexes, are not 
      included in the spores. The behaviour of nucleoli in meiotic mutants and a strain 
      with extrachromosomal rDNA suggests that they are not actively extruded but 
      rather are lost due to their detachment from the separating chromosomes. We 
      discuss the possibility that the exclusion of the nucleolus from the spores 
      serves the disposal of agents that resort to the nucleolus, and has a role in 
      spore dormancy or rejuvenation.
FAU - Fuchs, Jorg
AU  - Fuchs J
AD  - Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.
FAU - Loidl, Josef
AU  - Loidl J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Cell Nucleolus/metabolism/*ultrastructure
MH  - DNA, Ribosomal/analysis
MH  - In Situ Hybridization, Fluorescence
MH  - *Meiosis
MH  - *Mitosis
MH  - Nuclear Pore/metabolism
MH  - Nucleolus Organizer Region/*ultrastructure
MH  - Saccharomyces cerevisiae/cytology/genetics/*ultrastructure
MH  - Spores, Fungal/metabolism
EDAT- 2004/07/15 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/07/15 05:00
PHST- 2004/07/15 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/07/15 05:00 [entrez]
AID - 5275229 [pii]
AID - 10.1023/B:CHRO.0000034726.05374.db [doi]
PST - ppublish
SO  - Chromosome Res. 2004;12(5):427-38. doi: 10.1023/B:CHRO.0000034726.05374.db.